Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613120316_y3m8 TGTCAGTTTTTCTCTTTAGTGAAATGATACAG(+AGGACA)GTACCAGCAAAGTGACAATATGTCCTTTGAT NGG 10 8 17 8 24 0 100 5 10
    240613120315_6qbi TGGACATCAAAGGACATATTGTCACTTTGCTG(+AGGACA)GTACCTGTATCATTTCACTAAAGAGAAAAAC NGG 10 8 17 8 24 0 100 5 10
    240613120313_j6eu AGTCCTCTAGTCTTGGCTGTGGGGGCTTCCAG(+AGGACA)GTACCACTTGGGGAGAGGACTCAAGTAAGAC NGG 10 8 17 8 24 0 100 5 10
    240613120312_ugsh CACTTGTCTTACTTGAGTCCTCTCCCCAAGTG(+AGGACA)GTACCTGGAAGCCCCCACAGCCAAGACTAGA NGG 10 8 17 8 24 0 100 5 10
    240613120311_gz3n GGGTTGGGAACAAGGGGGTGGGGTCATCAGAG(+AGGACA)GTACCTGTTACTTTAGGGACCCTCTCTGTCT NGG 10 8 17 8 24 0 100 5 10
    240613120310_2bgl CTCTCAGACAGAGAGGGTCCCTAAAGTAACAG(+AGGACA)GTACCTCTGATGACCCCACCCCCTTGTTCCC NGG 10 8 17 8 24 0 100 5 10
    240613120308_9ydm CAGACTGGAGTTTTCTGCCTGTTTGTCTCTCG(+AGGACA)GATCCTCAGAATAGCCGTGTGATGTGAGGTA NGG 10 8 17 8 24 0 100 5 10
    240613120307_yim8 CTGCCTACCTCACATCACACGGCTATTCTGAG(+AGGACA)GATCCGAGAGACAAACAGGCAGAAAACTCCA NGG 10 8 17 8 24 0 100 5 10
    240613120306_jyen CTCATGACTTTATATATTCAAAGCTTTTAATG(+AGGACA)GTACCTGTTACACAGCAAGTACTCTGTGAAT NGG 10 8 17 8 24 0 100 5 10
    240613120305_yw6s TCAGCATTCACAGAGTACTTGCTGTGTAACAG(+AGGACA)GTACCATTAAAAGCTTTGAATATATAAAGTC NGG 10 8 17 8 24 0 100 5 10