Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613120342_23bo GGCAAAATAAATAGTTAAAACAAGTGATGCAG(+AGGACA)GATCCCATTTCATGCTCATGATCCCATTAAA NGG 10 8 17 8 24 0 100 5 10
    240613120340_d5k6 AATTCTTTAATGGGATCATGAGCATGAAATGG(+AGGACA)GATCCTGCATCACTTGTTTTAACTATTTATT NGG 10 8 17 8 24 0 100 5 10
    240613120339_x6we GTTCAGAAGGGATCAAAATTGCCATCTAACAG(+AGGACA)GTACCTTAAAGGATTCCTGGGCATCTACTTC NGG 10 8 17 8 24 0 100 5 10
    240613120338_htcj TTGTGGAAGTAGATGCCCAGGAATCCTTTAAG(+AGGACA)GTACCTGTTAGATGGCAATTTTGATCCCTTC NGG 10 8 17 8 24 0 100 5 10
    240613120336_5zuj TTGGGTTGATTTTCCTGAATTGGTTCCTATAG(+AGGACA)GATCCTCCAATTTTGGATCTTCTACAGATCC NGG 10 8 17 8 24 0 100 5 10
    240613120335_fsiq CTGGGGGATCTGTAGAAGATCCAAAATTGGAG(+AGGACA)GATCCTATAGGAACCAATTCAGGAAAATCAA NGG 10 8 17 8 24 0 100 5 10
    240613120334_mlpd TGAGGCACAGTCTCTGTTCTGGAATTCACAAG(+AGGACA)GTACCTGTAATAAACATCCAAACAAGAGTCA NGG 10 8 17 8 24 0 100 5 10
    240613120332_cdo8 ATTTGTGACTCTTGTTTGGATGTTTATTACAG(+AGGACA)GTACCTTGTGAATTCCAGAACAGAGACTGTG NGG 10 8 17 8 24 0 100 5 10
    240613120331_zxyz TCTAATGTAATAATGTTTTTTAAAGCACTCTG(+AGGACA)GTACCTACATGCCATCAATTCCACTTTCTTC NGG 10 8 17 8 24 0 100 5 10
    240613120330_wim5 CTTACGAAGAAAGTGGAATTGATGGCATGTAG(+AGGACA)GTACCAGAGTGCTTTAAAAAACATTATTACA NGG 10 8 17 8 24 0 100 5 10