Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613120439_srak TATAATAAAATAATACTAAAAGAGAGCATGTG(+AGGACA)GATCCATACACCAGATTAATGCGGGGATAGG NGG 10 8 17 8 24 0 100 5 10
    240613120438_yxlq CATACCCTATCCCCGCATTAATCTGGTGTATG(+AGGACA)GATCCACATGCTCTCTTTTAGTATTATTTTA NGG 10 8 17 8 24 0 100 5 10
    240613120436_905f CGACAAACGTGCATTTGATAGCACTATTTTAG(+AGGACA)GTACCCAGAAGCAGACAATACAGCAGTGAAT NGG 10 8 17 8 24 0 100 5 10
    240613120435_40c3 GCCATATTCACTGCTGTATTGTCTGCTTCTGG(+AGGACA)GTACCTAAAATAGTGCTATCAAATGCACGTT NGG 10 8 17 8 24 0 100 5 10
    240613120433_zs5q CGTCTACTTAAGCTATGTCCAGAGGCGTGCTG(+AGGACA)GATCCTGCAGAGAAACAGTATGGCTTTATGA NGG 10 8 17 8 24 0 100 5 10
    240613120432_ejae GAGTTTCATAAAGCCATACTGTTTCTCTGCAG(+AGGACA)GATCCAGCACGCCTCTGGACATAGCTTAAGT NGG 10 8 17 8 24 0 100 5 10
    240613120430_yx10 GGGGGGGCACGGAGGAAAGCAAGGCTGTGTGG(+AGGACA)GATCCACTGGAGCCTCAGGAGGAAGGGAACA NGG 10 8 17 8 24 0 100 5 10
    240613120429_p3ua CCCAGTGTTCCCTTCCTCCTGAGGCTCCAGTG(+AGGACA)GATCCCACACAGCCTTGCTTTCCTCCGTGCC NGG 10 8 17 8 24 0 100 5 10
    240613120427_y0jc GTATTGGTGCAGAGGAGAGCTCTTGGTGCTGG(+AGGACA)GATCCTATGCCACTCCCCACTCCATCTCTAA NGG 10 8 17 8 24 0 100 5 10
    240613120426_c0up TCTGTTTAGAGATGGAGTGGGGAGTGGCATAG(+AGGACA)GATCCCAGCACCAAGAGCTCTCCTCTGCACC NGG 10 8 17 8 24 0 100 5 10