Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613120507_80ym TCAAAGCCTTCTGAGACCCCATCCCACACGAG(+AGGACA)GTACCGTGAAGGGGCACTAAAGTGCATTCTG NGG 10 8 17 8 24 0 100 5 10
    240613120506_slhh TCCTTCAGAATGCACTTTAGTGCCCCTTCACG(+AGGACA)GTACCTCGTGTGGGATGGGGTCTCAGAAGGC NGG 10 8 17 8 24 0 100 5 10
    240613120505_snt0 CTTGAGCATCATACTGCTTTTAAATCCATGGG(+AGGACA)GATCCTGGAAGAGCTCCCCTACAGATACTGA NGG 10 8 17 8 24 0 100 5 10
    240613120503_r0vt GCCCCTCAGTATCTGTAGGGGAGCTCTTCCAG(+AGGACA)GATCCCCATGGATTTAAAAGCAGTATGATGC NGG 10 8 17 8 24 0 100 5 10
    240613120502_n238 AATAAAGCTGGACTGAAAGAAACATCTCTGAG(+AGGACA)GATCCAGGAAGTGGACTGTCATTCACCAAAT NGG 10 8 17 8 24 0 100 5 10
    240613120500_vnj6 AGTTGATTTGGTGAATGACAGTCCACTTCCTG(+AGGACA)GATCCTCAGAGATGTTTCTTTCAGTCCAGCT NGG 10 8 17 8 24 0 100 5 10
    240613120458_mpcm TAATTTTTTGTAACTTTCCACTACTCTACCTG(+AGGACA)GTACCATATCTGTGGATTTTGGCCAATATTA NGG 10 8 17 8 24 0 100 5 10
    240613120457_avxj AGTTTTAATATTGGCCAAAATCCACAGATATG(+AGGACA)GTACCAGGTAGAGTAGTGGAAAGTTACAAAA NGG 10 8 17 8 24 0 100 5 10
    240613120455_pjoj TGTGGCAGGGTGGATAATGCGTGAGGACTTGG(+AGGACA)GATCCTGATGGACTTTTAATTGTACCCACAA NGG 10 8 17 8 24 0 100 5 10
    240613120454_jl54 TTGAGTTGTGGGTACAATTAAAAGTCCATCAG(+AGGACA)GATCCCAAGTCCTCACGCATTATCCACCCTG NGG 10 8 17 8 24 0 100 5 10