Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613120524_qdzu CAAGACCTTCAAGAAGGTGGGCTCCGGGGATG(+AGGACA)GTACCATTGCTCTTTCCTAGCTAACCCTAGA NGG 10 8 17 8 24 0 100 5 10
    240613120522_rwhj CCGTATCTAGGGTTAGCTAGGAAAGAGCAATG(+AGGACA)GTACCATCCCCGGAGCCCACCTTCTTGAAGG NGG 10 8 17 8 24 0 100 5 10
    240613120521_xs1j AAATGGTCCATATAAATTTGTGGCATCAATGG(+AGGACA)GATCCCAACTTGGCTTCCCTAGTCTTCCTTC NGG 10 8 17 8 24 0 100 5 10
    240613120519_d46p CGTGGGAAGGAAGACTAGGGAAGCCAAGTTGG(+AGGACA)GATCCCATTGATGCCACAAATTTATATGGAC NGG 10 8 17 8 24 0 100 5 10
    240613120518_i3rs AGGACCATGACAAAGCCCCTGAGATCAAGGTG(+AGGACA)GTACCAGTTGCAGCAGCAATGTGGTGCTCAG NGG 10 8 17 8 24 0 100 5 10
    240613120516_uvcq TAAGGCTGAGCACCACATTGCTGCTGCAACTG(+AGGACA)GTACCACCTTGATCTCAGGGGCTTTGTCATG NGG 10 8 17 8 24 0 100 5 10
    240613120515_oca8 TGATGTGACTAGAAAGGCCTGGCCACTTTAGG(+AGGACA)GTACCAAGGGAGAACTCCAAGGAGCATTTTT NGG 10 8 17 8 24 0 100 5 10
    240613120512_za1a GGGGGAAAAATGCTCCTTGGAGTTCTCCCTTG(+AGGACA)GTACCCTAAAGTGGCCAGGCCTTTCTAGTCA NGG 10 8 17 8 24 0 100 5 10
    240613120510_v12o ATTGCCTTATGTAGTAATCAGCAACTGAAGGG(+AGGACA)GTACCCAACCTCTACAGTCTTCCAGCACAAT NGG 10 8 17 8 24 0 100 5 10
    240613120509_o6p3 GGGCAATTGTGCTGGAAGACTGTAGAGGTTGG(+AGGACA)GTACCCCTTCAGTTGCTGATTACTACATAAG NGG 10 8 17 8 24 0 100 5 10