Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613120538_d5os AATTATTCAATCACCTGGAAAAAAAAGTCTTG(+AGGACA)GTACCAATAAAATATGTTTTTCTGTCATCTT NGG 10 8 17 8 24 0 100 5 10
    240613120537_5p79 GAAAGAAGATGACAGAAAAACATATTTTATTG(+AGGACA)GTACCAAGACTTTTTTTTCCAGGTGATTGAA NGG 10 8 17 8 24 0 100 5 10
    240613120535_duvl TAAAATCAAATGAAGGTTTAAGATCACATAAG(+AGGACA)GATCCTGCTTCTGTTAGTTAAGGAAACTGCT NGG 10 8 17 8 24 0 100 5 10
    240613120534_72ah CTTAGAGCAGTTTCCTTAACTAACAGAAGCAG(+AGGACA)GATCCTTATGTGATCTTAAACCTTCATTTGA NGG 10 8 17 8 24 0 100 5 10
    240613120533_3atk TGATGCAGCTGACTGATGAAGGTACTGGTGAG(+AGGACA)GTACCTCTAGGGCTAGGACCAGATGCAGTGA NGG 10 8 17 8 24 0 100 5 10
    240613120532_2sjf TGAAGTCACTGCATCTGGTCCTAGCCCTAGAG(+AGGACA)GTACCTCACCAGTACCTTCATCAGTCAGCTG NGG 10 8 17 8 24 0 100 5 10
    240613120530_tkxw GTCTTCTTCTCTGCCAGATAACAGAGCCATTG(+AGGACA)GATCCACTTATTAAAGACTTCTTATCACTTA NGG 10 8 17 8 24 0 100 5 10
    240613120529_k9sa AATGTTAAGTGATAAGAAGTCTTTAATAAGTG(+AGGACA)GATCCAATGGCTCTGTTATCTGGCAGAGAAG NGG 10 8 17 8 24 0 100 5 10
    240613120528_feiu CTTTTTCTAGAGTCCTTTCTACACACACTTTG(+AGGACA)GTACCCTGGGGAGTTTGGATGCAACCAGGAA NGG 10 8 17 8 24 0 100 5 10
    240613120525_c1up CCTCCTTCCTGGTTGCATCCAAACTCCCCAGG(+AGGACA)GTACCAAAGTGTGTGTAGAAAGGACTCTAGA NGG 10 8 17 8 24 0 100 5 10