Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613120635_zjh9 GTTCCAGAAACCTTATTTGCCAAGAGGCTGCG(+AGGACA)GTACCTTTTTTTTTTTTTTTTTTCCAATCCT NGG 10 8 17 8 24 0 100 5 10
    240613120634_r3p4 AGCTCCTTGTCACCATGTTAAGAGATCATTTG(+AGGACA)GATCCTCTAATTAAGTTTAAAATTTGTTTTT NGG 10 8 17 8 24 0 100 5 10
    240613120633_sibg AAGGAAAAAACAAATTTTAAACTTAATTAGAG(+AGGACA)GATCCAAATGATCTCTTAACATGGTGACAAG NGG 10 8 17 8 24 0 100 5 10
    240613120632_9cnq GATGTTTACATTTACCATGTTCCCCTTATTAG(+AGGACA)GATCCGCACAGCCAAGTTCCGGGCCTCTTCC NGG 10 8 17 8 24 0 100 5 10
    240613120631_ctvg CCTGAGGAAGAGGCCCGGAACTTGGCTGTGCG(+AGGACA)GATCCTAATAAGGGGAACATGGTAAATGTAA NGG 10 8 17 8 24 0 100 5 10
    240613120629_m89x CCACACACACACACACACGGGCCAGTCAGACG(+AGGACA)GATCCAGGTGTCCAGTTGTGGCTGATCAGCC NGG 10 8 17 8 24 0 100 5 10
    240613120627_6evk CCCGAGGCTGATCAGCCACAACTGGACACCTG(+AGGACA)GATCCGTCTGACTGGCCCGTGTGTGTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240613120626_wafr TTAGGGCCCAAGAGACGGCCCAGCAGGTAAAG(+AGGACA)GTACCTGCTGCCAGGTCTAATGAGCTAAGTT NGG 10 8 17 8 24 0 100 5 10
    240613120625_5ys7 AATCAAACTTAGCTCATTAGACCTGGCAGCAG(+AGGACA)GTACCTTTACCTGCTGGGCCGTCTCTTGGGC NGG 10 8 17 8 24 0 100 5 10
    240613120623_868v ACATGTGGTTGCCATGGCTTAATGTATGGACG(+AGGACA)GATCCTCCACTGTCTTGCAATTCATTTGTTG NGG 10 8 17 8 24 0 100 5 10