Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613120453_qox5 AATCCTCTCTACTTGACTCAGGGGCTCACAAG(+AGGACA)GTACCATTATATGCAAAAGTACTGTATATTT NGG 10 8 17 8 24 0 100 5 10
    240613120452_9ong AGGAAAAATATACAGTACTTTTGCATATAATG(+AGGACA)GTACCTTGTGAGCCCCTGAGTCAAGTAGAGA NGG 10 8 17 8 24 0 100 5 10
    240613120450_jour CTAAATTTAAAAGTCTTAATGAATCTCCTTTG(+AGGACA)GATCCCTGAATGGTCCAAGATCTTGGAGTCT NGG 10 8 17 8 24 0 100 5 10
    240613120448_npib GCTCCAGACTCCAAGATCTTGGACCATTCAGG(+AGGACA)GATCCAAAGGAGATTCATTAAGACTTTTAAA NGG 10 8 17 8 24 0 100 5 10
    240613120447_phib AAAGTATGAATGTGTTCTGCACAGCAAACTGG(+AGGACA)GATCCTTATCAGTACAACCTGAAGACTAGAG NGG 10 8 17 8 24 0 100 5 10
    240613120445_3tuy AGGGACTCTAGTCTTCAGGTTGTACTGATAAG(+AGGACA)GATCCCAGTTTGCTGTGCAGAACACATTCAT NGG 10 8 17 8 24 0 100 5 10
    240613120444_ksgs AGAATTATCAAGGAACACCTAAAAATGACCAG(+AGGACA)GATCCAAATAGGTTATTTATATTATATTTCT NGG 10 8 17 8 24 0 100 5 10
    240613120443_knz0 TATAGAGAAATATAATATAAATAACCTATTTG(+AGGACA)GATCCTGGTCATTTTTAGGTGTTCCTTGATA NGG 10 8 17 8 24 0 100 5 10
    240613120441_arng AGCTGAGTTTTGCATAATGATTTTGTATCTTG(+AGGACA)GTACCTCACTGAATTCATTTATGATTTATGA NGG 10 8 17 8 24 0 100 5 10
    240613120440_ndil ATCCATCATAAATCATAAATGAATTCAGTGAG(+AGGACA)GTACCAAGATACAAAATCATTATGCAAAACT NGG 10 8 17 8 24 0 100 5 10