Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613120412_k8qd AATATGAGGGAGGCACATTTTCTAGTGGCTGG(+AGGACA)GATCCTCTTCCAAACCATAAGAAAAAAGGCA NGG 10 8 17 8 24 0 100 5 10
    240613120411_a4fl ACCACTGCCTTTTTTCTTATGGTTTGGAAGAG(+AGGACA)GATCCCAGCCACTAGAAAATGTGCCTCCCTC NGG 10 8 17 8 24 0 100 5 10
    240613120408_u8lo ACAGGGTTAAAGTTTTGGTCCTCAGCCCATTG(+AGGACA)GTACCATTAGGAAACAGGGTCTAGTCAGAGA NGG 10 8 17 8 24 0 100 5 10
    240613120407_s0i3 GAAATTCTCTGACTAGACCCTGTTTCCTAATG(+AGGACA)GTACCAATGGGCTGAGGACCAAAACTTTAAC NGG 10 8 17 8 24 0 100 5 10
    240613120406_bn8e CGGACTAGGTGGTTAGTGGATACTATTGTTTG(+AGGACA)GATCCTGACTGTTACAGGGTTAAAGTTTTGG NGG 10 8 17 8 24 0 100 5 10
    240613120405_gy0o GAGGACCAAAACTTTAACCCTGTAACAGTCAG(+AGGACA)GATCCAAACAATAGTATCCACTAACCACCTA NGG 10 8 17 8 24 0 100 5 10
    240613120402_3sfo GAGAAAGCCTAGAGTCTCCCCTTACTCCAGGG(+AGGACA)GTACCAGCTTCAGAAGAAGGTGTGAGCTCAC NGG 10 8 17 8 24 0 100 5 10
    240613120401_xzxy GAAGTGTGAGCTCACACCTTCTTCTGAAGCTG(+AGGACA)GTACCCCTGGAGTAAGGGGAGACTCTAGGCT NGG 10 8 17 8 24 0 100 5 10
    240613120359_2x1j CCTGGTATACCGGGAAGGTGGTCTAGCCTAGG(+AGGACA)GTACCAGGGCTGCAGGTGAAACCTGAACTTT NGG 10 8 17 8 24 0 100 5 10
    240613120356_5vdp GGGTAAAAGTTCAGGTTTCACCTGCAGCCCTG(+AGGACA)GTACCCTAGGCTAGACCACCTTCCCGGTATA NGG 10 8 17 8 24 0 100 5 10