Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613120303_0on0 TAACCAATTGCTTAGGGGCAGGAAGATGTGGG(+AGGACA)GATCCCACCTTGCCAGAGCTCTTACCATCTT NGG 10 8 17 8 24 0 100 5 10
    240613120301_zd13 TGGCAAAGATGGTAAGAGCTCTGGCAAGGTGG(+AGGACA)GATCCCCACATCTTCCTGCCCCTAAGCAATT NGG 10 8 17 8 24 0 100 5 10
    240613120300_5q6k TAGGCTCACACACTAAGATTGACTGGCATATG(+AGGACA)GATCCCTTTCTTAGATGGTGGTGTATAGCTT NGG 10 8 17 8 24 0 100 5 10
    240613120258_wzvx TCTTGAAGCTATACACCACCATCTAAGAAAGG(+AGGACA)GATCCATATGCCAGTCAATCTTAGTGTGTGA NGG 10 8 17 8 24 0 100 5 10
    240613120257_yf6a ATTTTAAATTTTTTACCAAGCACATTCTGAAG(+AGGACA)GTACCAACAAATGACATTATCTACTTCCTAC NGG 10 8 17 8 24 0 100 5 10
    240613120255_9n8v TACTAGTAGGAAGTAGATAATGTCATTTGTTG(+AGGACA)GTACCTTCAGAATGTGCTTGGTAAAAAATTT NGG 10 8 17 8 24 0 100 5 10
    240613120254_qppp TCTAAGAGTTCTGTGTATATTTCTTCTAGTTG(+AGGACA)GTACCTACACAAATTAGATTTCTCTTGCTTT NGG 10 8 17 8 24 0 100 5 10
    240613120253_x5u1 TGGCAAAAGCAAGAGAAATCTAATTTGTGTAG(+AGGACA)GTACCAACTAGAAGAAATATACACAGAACTC NGG 10 8 17 8 24 0 100 5 10
    240613120251_uo6b GTAAATTAACAACTCAGTTATTAAACTAGCAG(+AGGACA)GATCCTACAATGATACATCTAGTGAAAGTAG NGG 10 8 17 8 24 0 100 5 10
    240613120250_kjxd TAACTCTACTTTCACTAGATGTATCATTGTAG(+AGGACA)GATCCTGCTAGTTTAATAACTGAGTTGTTAA NGG 10 8 17 8 24 0 100 5 10