Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613115050_k8i3 ACAGGTCCAACAGAGTATCTCAGACATGGGTG(+AGGACA)GTACCACCACAGCTCTGGGGAGCCTCTGACC NGG 10 8 17 8 24 0 100 5 10
    240613115049_id7a AGGAGGAGGGTTTTTTTTTTAAGACAAAGATG(+AGGACA)GTACCATGGTATCAGAAAATAGTGTATAAAT NGG 10 8 17 8 24 0 100 5 10
    240613115048_z4c0 AAGGAATTTATACACTATTTTCTGATACCATG(+AGGACA)GTACCATCTTTGTCTTAAAAAAAAAACCCTC NGG 10 8 17 8 24 0 100 5 10
    240613115047_zanb TATTATAGCTACTGCTGCCGCTACTGTTGTTG(+AGGACA)GTACCACCTCAGATACATAGAGGGACACATA NGG 10 8 17 8 24 0 100 5 10
    240613115045_s2da TGATTTATGTGTCCCTCTATGTATCTGAGGTG(+AGGACA)GTACCAACAACAGTAGCGGCAGCAGTAGCTA NGG 10 8 17 8 24 0 100 5 10
    240613115044_k1kg CGTCTGCTTCCCTCTCTCCTCGTGCTAAGCTG(+AGGACA)GATCCGTGTCCTTTTCTTTTTAACGAGTTCA NGG 10 8 17 8 24 0 100 5 10
    240613115043_c35w TCACTTGAACTCGTTAAAAAGAAAAGGACACG(+AGGACA)GATCCAGCTTAGCACGAGGAGAGAGGGAAGC NGG 10 8 17 8 24 0 100 5 10
    240613115042_s4ad GCTCACCCGCAAGGGGTTCCAGAACAAATCTG(+AGGACA)GTACCCACGAGGACAAGCCAAACCAAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240613115040_apyb CCTGATCCACTTGGTTTGGCTTGTCCTCGTGG(+AGGACA)GTACCAGATTTGTTCTGGAACCCCTTGCGGG NGG 10 8 17 8 24 0 100 5 10
    240613115038_b2sn AAGAGAAGTAGGAGGTTGACTGACCAACTTGG(+AGGACA)GTACCATAGGATTTTGTTTATTGCATAGGTC NGG 10 8 17 8 24 0 100 5 10