Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613115037_xbk3 TAGATGACCTATGCAATAAACAAAATCCTATG(+AGGACA)GTACCCAAGTTGGTCAGTCAACCTCCTACTT NGG 10 8 17 8 24 0 100 5 10
    240613115034_tauq GCTATGCACCCCTCTCCCTGGCACTGACCCTG(+AGGACA)GATCCCCATCTTCTGTACCCAGTGACTAGAC NGG 10 8 17 8 24 0 100 5 10
    240613115032_dn44 CAGTCGTCTAGTCACTGGGTACAGAAGATGGG(+AGGACA)GATCCAGGGTCAGTGCCAGGGAGAGGGGTGC NGG 10 8 17 8 24 0 100 5 10
    240613115030_x2lr TTCCTGCCAAACTACCTTGCGAGGTTTCCTGG(+AGGACA)GTACCCAAGTGGAGCCAAAGCTCACTAGGAC NGG 10 8 17 8 24 0 100 5 10
    240613115028_xmti GTCCTGTCCTAGTGAGCTTTGGCTCCACTTGG(+AGGACA)GTACCCAGGAAACCTCGCAAGGTAGTTTGGC NGG 10 8 17 8 24 0 100 5 10
    240613115027_5pmi CATGGAGAGACTTAATAAAGGGTTTTACAAGG(+AGGACA)GTACCTGGATCCCAGGGGGAAGTAGTTTATC NGG 10 8 17 8 24 0 100 5 10
    240613115025_8atq CCAAGGATAAACTACTTCCCCCTGGGATCCAG(+AGGACA)GTACCCTTGTAAAACCCTTTATTAAGTCTCT NGG 10 8 17 8 24 0 100 5 10
    240613115023_vhoy AGACTTAATAAAGGGTTTTACAAGGGTACCTG(+AGGACA)GATCCCAGGGGGAAGTAGTTTATCCTTGGGG NGG 10 8 17 8 24 0 100 5 10
    240613115021_dsul CTGTGCCCCAAGGATAAACTACTTCCCCCTGG(+AGGACA)GATCCAGGTACCCTTGTAAAACCCTTTATTA NGG 10 8 17 8 24 0 100 5 10
    240613115019_ukyr TCTCAAAGATGAGGCCCACATGAACCTGCTGG(+AGGACA)GATCCCACGAACACATATTGGACCTGAGCAC NGG 10 8 17 8 24 0 100 5 10