Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613114931_ywdr TTCCTTGAAGTTTTAGGTCCCCTTGAAATTAG(+AGGACA)GATCCGTCACACTTTCACGGACAGAGCCTAG NGG 10 8 17 8 24 0 100 5 10
    240613114930_h8ak TAAAGCCCCCTACTGCACCCCTGACCTGTGAG(+AGGACA)GATCCATTTTTTTTTCTTTCCTTCCCCAAAT NGG 10 8 17 8 24 0 100 5 10
    240613114929_zhm1 CCGAGATTTGGGGAAGGAAAGAAAAAAAAATG(+AGGACA)GATCCTCACAGGTCAGGGGTGCAGTAGGGGG NGG 10 8 17 8 24 0 100 5 10
    240613114927_tq5p CAGAAGTTAAGGTGGCCACAGCACAGGTCAGG(+AGGACA)GATCCAAGGAGCTGGGGAGGACTGCTCAAAA NGG 10 8 17 8 24 0 100 5 10
    240613114925_8233 ACTAGTTTTGAGCAGTCCTCCCCAGCTCCTTG(+AGGACA)GATCCCTGACCTGTGCTGTGGCCACCTTAAC NGG 10 8 17 8 24 0 100 5 10
    240613114923_mbl3 TCTAACTCCATTGGGGACAGGCTAGGGGATGG(+AGGACA)GATCCTCTACCTTTTTCAGGCCTCTGCAGAC NGG 10 8 17 8 24 0 100 5 10
    240613114922_bjug CTGGTGTCTGCAGAGGCCTGAAAAAGGTAGAG(+AGGACA)GATCCCATCCCCTAGCCTGTCCCCAATGGAG NGG 10 8 17 8 24 0 100 5 10
    240613114920_1qzm TCTGTGGTGCAGACCATAAAAGGAAGCCATTG(+AGGACA)GTACCTTCTCAGGTACTTAGCTTTTTGGATA NGG 10 8 17 8 24 0 100 5 10
    240613114919_lie7 CCTGATATCCAAAAAGCTAAGTACCTGAGAAG(+AGGACA)GTACCAATGGCTTCCTTTTATGGTCTGCACC NGG 10 8 17 8 24 0 100 5 10
    240613114918_b6tb ATGCAGGAGCACCCCTTTACCCATTTTAAAGG(+AGGACA)GTACCTTGTACATTTATTTAATCCTACTAAT NGG 10 8 17 8 24 0 100 5 10