Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613115010_ni4a TGGGGGCTCTTAAGGATTTCCCAGAAAAACAG(+AGGACA)GATCCTGGGATGGGACGTGGTTGCTCGGGCA NGG 10 8 17 8 24 0 100 5 10
    240613115008_vjlz TTTTTTGCCCGAGCAACCACGTCCCATCCCAG(+AGGACA)GATCCTGTTTTTCTGGGAAATCCTTAAGAGC NGG 10 8 17 8 24 0 100 5 10
    240613115006_yaou ACCACACATACCTTGAGGAGGACTGCCCTAGG(+AGGACA)GTACCAGGTTTTATTCAGCTCTAAGTCCCAG NGG 10 8 17 8 24 0 100 5 10
    240613115004_rpca TTACTCTGGGACTTAGAGCTGAATAAAACCTG(+AGGACA)GTACCCTAGGGCAGTCCTCCTCAAGGTATGT NGG 10 8 17 8 24 0 100 5 10
    240613115003_zup5 TATGTGTGGGGATCCCTTCACCCACCATACTG(+AGGACA)GTACCCCTTCTTCAGCTCCATCTGTCCCTAT NGG 10 8 17 8 24 0 100 5 10
    240613115001_pwdy AGGGGATAGGGACAGATGGAGCTGAAGAAGGG(+AGGACA)GTACCAGTATGGTGGGTGAAGGGATCCCCAC NGG 10 8 17 8 24 0 100 5 10
    240613114959_vwwr CTTCTTCTTTCTCTAGTGCAAGTATGTGTGGG(+AGGACA)GATCCCTTCACCCACCATACTGGTACCCCTT NGG 10 8 17 8 24 0 100 5 10
    240613114957_2p5t TGAAGAAGGGGTACCAGTATGGTGGGTGAAGG(+AGGACA)GATCCCCACACATACTTGCACTAGAGAAAGA NGG 10 8 17 8 24 0 100 5 10
    240613114955_stx8 TATTTGCTGTGCAAAGCACGCCCTAAGATGGG(+AGGACA)GTACCTTTGATCTCACCGTGCGGCTGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240613114954_7o2i TTGAATCCTGCAGCCGCACGGTGAGATCAAAG(+AGGACA)GTACCCCATCTTAGGGCGTGCTTTGCACAGC NGG 10 8 17 8 24 0 100 5 10