Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613114846_xgvz TTACGGTGCTAGAGTGGGATCTTCTGTGTTAG(+AGGACA)GATCCTTCTGGGGAAGCAGAATGAGTGTAGG NGG 10 8 17 8 24 0 100 5 10
    240613114844_exk3 TATTGAATTTCTTCAGCAGCCAATGAATCCTG(+AGGACA)GATCCATACAAAAATATATTTAGCTTCCACC NGG 10 8 17 8 24 0 100 5 10
    240613114843_s7uq TTGCAGGTGGAAGCTAAATATATTTTTGTATG(+AGGACA)GATCCAGGATTCATTGGCTGCTGAAGAAATT NGG 10 8 17 8 24 0 100 5 10
    240613114841_w821 GAAGATGTTAAGGGAGGGTAGTTAAATCATGG(+AGGACA)GTACCTAAGCTGGGGTTTTAGATTGAAGATG NGG 10 8 17 8 24 0 100 5 10
    240613114840_5mg4 TTGGGCATCTTCAATCTAAAACCCCAGCTTAG(+AGGACA)GTACCCATGATTTAACTACCCTCCCTTAACA NGG 10 8 17 8 24 0 100 5 10
    240613114839_0b64 TTGAGGTATTCGTTGGGGGAGTGATGGAGGAG(+AGGACA)GATCCAAAATGATTCATTGTATGGATGGCAG NGG 10 8 17 8 24 0 100 5 10
    240613114838_sbs8 CCTTTCTGCCATCCATACAATGAATCATTTTG(+AGGACA)GATCCTCCTCCATCACTCCCCCAACGAATAC NGG 10 8 17 8 24 0 100 5 10
    240613114836_14ww TTATACCACTAAGAAACCATGTACATAAATGG(+AGGACA)GATCCAAATATAGTTTGGAGACAACCCAACT NGG 10 8 17 8 24 0 100 5 10
    240613114835_7k6n ATATTAGTTGGGTTGTCTCCAAACTATATTTG(+AGGACA)GATCCCATTTATGTACATGGTTTCTTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240613114833_du04 AGAAACAGATTTGTATTGTGTTTTATGGCCTG(+AGGACA)GATCCAGGAGTACTTTTATAATGGGTAAGAT NGG 10 8 17 8 24 0 100 5 10