Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613114744_xuvj ATCCTGGTTCCTCTTGCCTGCTTAAAAACTGG(+AGGACA)GATCCCGACTCCATGGATCAAAACTGAGTGA NGG 10 8 17 8 24 0 100 5 10
    240613114743_3al1 CCAGCACTTTTGTTCACTTAAAATGTTGGAAG(+AGGACA)GATCCAGGCCCTTTTCAGGAAGAAGCTGCCC NGG 10 8 17 8 24 0 100 5 10
    240613114741_ih3s TCTGGGGGCAGCTTCTTCCTGAAAAGGGCCTG(+AGGACA)GATCCTTCCAACATTTTAAGTGAACAAAAGT NGG 10 8 17 8 24 0 100 5 10
    240613114739_qbj0 AAAAAAAAAACAGAAACAAAAAATGGTGCCAG(+AGGACA)GTACCACAAGAGTAACAGAACTGTGCTGTTT NGG 10 8 17 8 24 0 100 5 10
    240613114738_urkh CCAGAAAACAGCACAGTTCTGTTACTCTTGTG(+AGGACA)GTACCTGGCACCATTTTTTGTTTCTGTTTTT NGG 10 8 17 8 24 0 100 5 10
    240613114737_r3bd AGGCCACTTCTCCAAGACAGTTTCTGACACTG(+AGGACA)GTACCTTCCCCTAATCATGTATCTCCCAAAA NGG 10 8 17 8 24 0 100 5 10
    240613114736_xg7w TCATCTTTTGGGAGATACATGATTAGGGGAAG(+AGGACA)GTACCAGTGTCAGAAACTGTCTTGGAGAAGT NGG 10 8 17 8 24 0 100 5 10
    240613114735_u92f GCTTATCCAGGGCTCACTTTCGTCCTTTGTAG(+AGGACA)GTACCCGCATTCCTACGCATTCATATACATA NGG 10 8 17 8 24 0 100 5 10
    240613114733_nr6r GTTTGTATGTATATGAATGCGTAGGAATGCGG(+AGGACA)GTACCTACAAAGGACGAAAGTGAGCCCTGGA NGG 10 8 17 8 24 0 100 5 10
    240613114732_1box CAGATTTTAGGTAAGTTTATTTGACTAAATTG(+AGGACA)GATCCTTTTATATTAACAATTCAGCATCTTT NGG 10 8 17 8 24 0 100 5 10