Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613114731_h768 TTATAAAAGATGCTGAATTGTTAATATAAAAG(+AGGACA)GATCCAATTTAGTCAAATAAACTTACCTAAA NGG 10 8 17 8 24 0 100 5 10
    240613114729_vab4 ATTCCTGTCCCAGGATCCAAATCCCTCTTCAG(+AGGACA)GTACCAGGCACTGTGCAAAGACACATTCAGG NGG 10 8 17 8 24 0 100 5 10
    240613114727_7n3y TTTTGCCTGAATGTGTCTTTGCACAGTGCCTG(+AGGACA)GTACCTGAAGAGGGATTTGGATCCTGGGACA NGG 10 8 17 8 24 0 100 5 10
    240613114725_90hz AGCTCACAACTGTCTTGTTATTCCTGTCCCAG(+AGGACA)GATCCAAATCCCTCTTCAGGTACCAGGCACT NGG 10 8 17 8 24 0 100 5 10
    240613114724_yyj5 TGCACAGTGCCTGGTACCTGAAGAGGGATTTG(+AGGACA)GATCCTGGGACAGGAATAACAAGACAGTTGT NGG 10 8 17 8 24 0 100 5 10
    240613114721_4ftj TATATAATATCTTCTTTTTTTTTAAAGCCCCG(+AGGACA)GATCCAAGGCGTCCAAAGTTATTAAAATAAA NGG 10 8 17 8 24 0 100 5 10
    240613114719_xa6k TGAACTTTATTTTAATAACTTTGGACGCCTTG(+AGGACA)GATCCGGGGCTTTAAAAAAAAAGAAGATATT NGG 10 8 17 8 24 0 100 5 10
    240613114718_8ndv GTAAGATACATAAATATAGAATTAATATAAAG(+AGGACA)GTACCACTATGTTTTACTTATAATAAAAACA NGG 10 8 17 8 24 0 100 5 10
    240613114717_6etk AAATGTGTTTTTATTATAAGTAAAACATAGTG(+AGGACA)GTACCTTTATATTAATTCTATATTTATGTAT NGG 10 8 17 8 24 0 100 5 10
    240613114715_wsh9 CAACTGAGAGAAGTGGGAAGAGCATCTGGTGG(+AGGACA)GTACCAATTGGCAGAGAAGGGGAGAAGACTG NGG 10 8 17 8 24 0 100 5 10