Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613114628_hbq9 AGTGTGTATTCCCCGTCCCCTCTGACACCTGG(+AGGACA)GATCCAGATTTTGAATTGTGGCCCTGCTGGA NGG 10 8 17 8 24 0 100 5 10
    240613114627_8rru TTAAAAGAGTAGCTTCCTCTGCTGTACTAGTG(+AGGACA)GATCCTGTTTTTGTTGCTTCCAGGGACCAGC NGG 10 8 17 8 24 0 100 5 10
    240613114625_2b3n AAAAAGCTGGTCCCTGGAAGCAACAAAAACAG(+AGGACA)GATCCACTAGTACAGCAGAGGAAGCTACTCT NGG 10 8 17 8 24 0 100 5 10
    240613114623_swi0 TTTACATGAAGTGATGAGACCAAAGGCCCAAG(+AGGACA)GATCCCCGTCCCCACCCCCCACCCCCTTTTG NGG 10 8 17 8 24 0 100 5 10
    240613114621_m27c GAAAACAAAAGGGGGTGGGGGGTGGGGACGGG(+AGGACA)GATCCTTGGGCCTTTGGTCTCATCACTTCAT NGG 10 8 17 8 24 0 100 5 10
    240613114619_3d0e CAGAGCCAGTTTGGTCTTTCTCCCCCTGCCAG(+AGGACA)GATCCCAGGCCCTAATCAGTATTATTTCTTT NGG 10 8 17 8 24 0 100 5 10
    240613114617_3fsy TTATGAAAGAAATAATACTGATTAGGGCCTGG(+AGGACA)GATCCTGGCAGGGGGAGAAAGACCAAACTGG NGG 10 8 17 8 24 0 100 5 10
    240613114615_2fdl AGCTTCAGGGTCCTCCTCGCTGTCCACACATG(+AGGACA)GATCCCAGCGGAATCTCCGCTGACACGGAAG NGG 10 8 17 8 24 0 100 5 10
    240613114614_m9g0 TTTGCCTTCCGTGTCAGCGGAGATTCCGCTGG(+AGGACA)GATCCATGTGTGGACAGCGAGGAGGACCCTG NGG 10 8 17 8 24 0 100 5 10
    240613114612_xtlm TAGGCCCCTCCCAGCTGTGAGGGTCCAGGGAG(+AGGACA)GATCCTGTGGCCGAGTCTTCAGTGGCCGAGT NGG 10 8 17 8 24 0 100 5 10