Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613114537_nsxd AAACAAGCTTAGGGGACTGAGGGGAGCCGGGG(+AGGACA)GATCCACAGCACCCAAAAGGTGGAATAAGTT NGG 10 8 17 8 24 0 100 5 10
    240613114536_v02l AAAGGGGTTTTGATAGAAGGAAGAGACTTCCG(+AGGACA)GTACCATTGTAACTAAAGTGTCAGTCATTTT NGG 10 8 17 8 24 0 100 5 10
    240613114534_2piw TAGTTAAAATGACTGACACTTTAGTTACAATG(+AGGACA)GTACCGGAAGTCTCTTCCTTCTATCAAAACC NGG 10 8 17 8 24 0 100 5 10
    240613114533_u7o4 ACATCTCCTGCCTCAGTCTTCAAAGCTATTTG(+AGGACA)GATCCATAAGTTTGTTATATGCATCTTCTTG NGG 10 8 17 8 24 0 100 5 10
    240613114532_tx3e ACATGCAAGAAGATGCATATAACAAACTTATG(+AGGACA)GATCCAAATAGCTTTGAAGACTGAGGCAGGA NGG 10 8 17 8 24 0 100 5 10
    240613114531_0tt0 CAAGGTAATGAGTTTCGTGGTAGCATTTTGAG(+AGGACA)GATCCATGTCATCGTTCATTGTTCTTTTCCT NGG 10 8 17 8 24 0 100 5 10
    240613114530_68np GGGCTAGGAAAAGAACAATGAACGATGACATG(+AGGACA)GATCCTCAAAATGCTACCACGAAACTCATTA NGG 10 8 17 8 24 0 100 5 10
    240613114528_65v3 CTTGCAAAAGCCAAAGCTGCCTGAGTAGTCTG(+AGGACA)GATCCTGCAACATCACTTTGCTTCAGGAGTT NGG 10 8 17 8 24 0 100 5 10
    240613114527_g98x AAACTAACTCCTGAAGCAAAGTGATGTTGCAG(+AGGACA)GATCCAGACTACTCAGGCAGCTTTGGCTTTT NGG 10 8 17 8 24 0 100 5 10
    240613114526_mrcy CTCCAAAGCTAGAGTAATCATGAGGGAGGCAG(+AGGACA)GATCCACGGAGAATCACAAAGGATGCATTTT NGG 10 8 17 8 24 0 100 5 10