Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613114455_eo2z GATGTGGCCGCGGAACATGTTAGCCTTGACTG(+AGGACA)GATCCTTGAACAAAAGCAACGATAGAGATGA NGG 10 8 17 8 24 0 100 5 10
    240613114454_sp3z GATGCCCCCCCCCCAACCCAGTGTATTCTCTG(+AGGACA)GATCCCTAAGTGCCTTAGATCCTGAGCCACA NGG 10 8 17 8 24 0 100 5 10
    240613114452_qv5j AGCTCTGTGGCTCAGGATCTAAGGCACTTAGG(+AGGACA)GATCCAGAGAATACACTGGGTTGGGGGGGGG NGG 10 8 17 8 24 0 100 5 10
    240613114451_9es8 GGGTCCAGGCTCTTCAGGCTCAGTAGGGTCAG(+AGGACA)GTACCAGCTATACTCTGGTTGTTAAAGGCTT NGG 10 8 17 8 24 0 100 5 10
    240613114450_sybe ACCCCAAGCCTTTAACAACCAGAGTATAGCTG(+AGGACA)GTACCTGACCCTACTGAGCCTGAAGAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240613114449_c22h ATAACTGGCTGGGGGGTTAGGCAAAGGACCTG(+AGGACA)GTACCCTTACTACTCAAAGCCTACCTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240613114447_lzw1 ACTGCCCACTAGGTAGGCTTTGAGTAGTAAGG(+AGGACA)GTACCAGGTCCTTTGCCTAACCCCCCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240613114446_1zij ACCACGGCCTCTGAATGGGGCTGAGGCTCATG(+AGGACA)GATCCCGGAATGAGACCAGGATGCATAAGAC NGG 10 8 17 8 24 0 100 5 10
    240613114444_o5t7 CACTGGTCTTATGCATCCTGGTCTCATTCCGG(+AGGACA)GATCCATGAGCCTCAGCCCCATTCAGAGGCC NGG 10 8 17 8 24 0 100 5 10
    240613114442_1ljn GGCTTCTTGTGATCTCCTTTGCCAGTCACTGG(+AGGACA)GATCCAGAACAATCTACTTCTTCAAGCATGC NGG 10 8 17 8 24 0 100 5 10