Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613114517_28h4 TCATCCTATAGCTCAGGCTTCAAACTCACTGG(+AGGACA)GATCCGCCTGCTCTGCCTCCTTAGTGCTGGG NGG 10 8 17 8 24 0 100 5 10
    240613114516_5wl3 GGACAGAAGCAGTTTGAGCAGGTGTCTATATG(+AGGACA)GTACCCTGCATCCGGGGCCCTTTGGGAACTG NGG 10 8 17 8 24 0 100 5 10
    240613114514_71j4 CCGGCCAGTTCCCAAAGGGCCCCGGATGCAGG(+AGGACA)GTACCATATAGACACCTGCTCAAACTGCTTC NGG 10 8 17 8 24 0 100 5 10
    240613114513_a87w CACATTTCTAAAAATCTTTCAATGACAAGTTG(+AGGACA)GTACCCTTTAGCTAACTAAAGCTAAAAGGTG NGG 10 8 17 8 24 0 100 5 10
    240613114512_3ezx CCTTCCACCTTTTAGCTTTAGTTAGCTAAAGG(+AGGACA)GTACCAACTTGTCATTGAAAGATTTTTAGAA NGG 10 8 17 8 24 0 100 5 10
    240613114510_xnvc TCCTCCTATTCCTCCTGTGAGTTAGTGGCAGG(+AGGACA)GTACCAACCCACTGGAGGGCAACAGGGAGGG NGG 10 8 17 8 24 0 100 5 10
    240613114509_3epw CCACACCCTCCCTGTTGCCCTCCAGTGGGTTG(+AGGACA)GTACCCTGCCACTAACTCACAGGAGGAATAG NGG 10 8 17 8 24 0 100 5 10
    240613114507_4gnl GAAGGTCAGACAACAGCTTCCCTACTTGCCAG(+AGGACA)GTACCTGCACTGGGGGGCCCCGGGCCACAGG NGG 10 8 17 8 24 0 100 5 10
    240613114506_c7p9 CCAATCCTGTGGCCCGGGGCCCCCCAGTGCAG(+AGGACA)GTACCTGGCAAGTAGGGAAGCTGTTGTCTGA NGG 10 8 17 8 24 0 100 5 10
    240613114504_occ1 ATCCTGCCATGTGGCTGGTTACCTGTGCTCAG(+AGGACA)GTACCATCCGTCCATCTCCTCACATCTTCCC NGG 10 8 17 8 24 0 100 5 10