Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613114307_cnl6 CCACTGATGGCTTTTAAAATAAGCTGCAGATG(+AGGACA)GTACCTCTACGGCTTCCCTGTACTAGGCATT NGG 10 8 17 8 24 0 100 5 10
    240613114305_0avd TGAGCGGCTGCCGCCCCACCCTGTGCTCTCCG(+AGGACA)GATCCCACTAGCCACAGGCATCTCCAGGAGG NGG 10 8 17 8 24 0 100 5 10
    240613114303_6z9d CGGGCCCTCCTGGAGATGCCTGTGGCTAGTGG(+AGGACA)GATCCGGAGAGCACAGGGTGGGGCGGCAGCC NGG 10 8 17 8 24 0 100 5 10
    240613114302_12ug GCACCCAGATAGGCAAGGTCACCTTGACACTG(+AGGACA)GTACCATGGTAACCTTGGCTCTTTAGGGCTG NGG 10 8 17 8 24 0 100 5 10
    240613114300_fcst TGCACCAGCCCTAAAGAGCCAAGGTTACCATG(+AGGACA)GTACCAGTGTCAAGGTGACCTTGCCTATCTG NGG 10 8 17 8 24 0 100 5 10
    240613114259_xumu CTGTGCTACCACACCCAGCCTGAGTTTAAATG(+AGGACA)GTACCCTCGGACCCAAGGCAGCTCTGTGCTA NGG 10 8 17 8 24 0 100 5 10
    240613114257_e9u8 TGTCTTAGCACAGAGCTGCCTTGGGTCCGAGG(+AGGACA)GTACCATTTAAACTCAGGCTGGGTGTGGTAG NGG 10 8 17 8 24 0 100 5 10
    240613114256_g580 CTGTGCTACCACACCCAGCCTGAGTTTAAATG(+AGGACA)GTACCCTCGGACCCAAGGCAGCTCTGTGCTA NGG 10 8 17 8 24 0 100 5 10
    240613114254_0rsx TGTCTTAGCACAGAGCTGCCTTGGGTCCGAGG(+AGGACA)GTACCATTTAAACTCAGGCTGGGTGTGGTAG NGG 10 8 17 8 24 0 100 5 10
    240613114252_6d5y CTTCATTGGCATGGCAACAGGTAGGCATATGG(+AGGACA)GATCCTCCTAGTGGTTAGTACAATCACACCA NGG 10 8 17 8 24 0 100 5 10