Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613114205_cqr7 GGCTTTTGGAGCAATGGAGCACAGATGTCAAG(+AGGACA)GATCCATAAGGGTGTCTATGTATCCCAGGTC NGG 10 8 17 8 24 0 100 5 10
    240613114204_j9oe TCCCTGGCTCCACTATCGAACTGCTGGGTGGG(+AGGACA)GATCCGTTCCTGCTATTACCCTCTTCTACTG NGG 10 8 17 8 24 0 100 5 10
    240613114202_kkq9 CATCTCAGTAGAAGAGGGTAATAGCAGGAACG(+AGGACA)GATCCCCACCCAGCAGTTCGATAGTGGAGCC NGG 10 8 17 8 24 0 100 5 10
    240613114201_j6t0 GACCCTTGGGTGTTCAGAACTCCTGCCAGGAG(+AGGACA)GATCCTCAGTGCCCACTAAGTCCATGCCATC NGG 10 8 17 8 24 0 100 5 10
    240613114200_v1w6 CTGGGGATGGCATGGACTTAGTGGGCACTGAG(+AGGACA)GATCCTCCTGGCAGGAGTTCTGAACACCCAA NGG 10 8 17 8 24 0 100 5 10
    240613114159_78w5 TTCAATAAATACTTGTTGCATGAACAAACTTG(+AGGACA)GATCCAAATTTTTAGAAATATTGAATTTGGA NGG 10 8 17 8 24 0 100 5 10
    240613114157_q3nw GAGCATCCAAATTCAATATTTCTAAAAATTTG(+AGGACA)GATCCAAGTTTGTTCATGCAACAAGTATTTA NGG 10 8 17 8 24 0 100 5 10
    240613114156_japh GTGTCCATGTTTAAAGACATCATCCCTGCCAG(+AGGACA)GATCCATGAGAAAAAATATATTTTCTTACTC NGG 10 8 17 8 24 0 100 5 10
    240613114154_5mvv TGTGGGAGTAAGAAAATATATTTTTTCTCATG(+AGGACA)GATCCTGGCAGGGATGATGTCTTTAAACATG NGG 10 8 17 8 24 0 100 5 10
    240613114153_gq9j AGAGCAGCCTCAGAGAGGCTTGTCCTAGTCAG(+AGGACA)GATCCAGGATCTTGGCTGGGGCCTGCTTCTT NGG 10 8 17 8 24 0 100 5 10