Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613114151_0yb6 GAACAAAGAAGCAGGCCCCAGCCAAGATCCTG(+AGGACA)GATCCTGACTAGGACAAGCCTCTCTGAGGCT NGG 10 8 17 8 24 0 100 5 10
    240613114150_4m9c CCAGGCATGCTCCTGCCTAGGTGAAATGAAAG(+AGGACA)GATCCACATACTGGCACATGACATGATCCTA NGG 10 8 17 8 24 0 100 5 10
    240613114149_l6dl CTGAATAGGATCATGTCATGTGCCAGTATGTG(+AGGACA)GATCCTTTCATTTCACCTAGGCAGGAGCATG NGG 10 8 17 8 24 0 100 5 10
    240613114147_v541 CCTTTTAGCTGCCATCTGCCAAGCACACCTGG(+AGGACA)GATCCACCAGTTCTGGCAATGCTGTGACAGT NGG 10 8 17 8 24 0 100 5 10
    240613114145_k6uz CAGTCACTGTCACAGCATTGCCAGAACTGGTG(+AGGACA)GATCCCAGGTGTGCTTGGCAGATGGCAGCTA NGG 10 8 17 8 24 0 100 5 10
    240613114144_acgj AAAAAAAAAAAATTCCCGGGAGCAACTGCCTG(+AGGACA)GATCCTTTCCCTACAGTCGCTCGATCTCCCA NGG 10 8 17 8 24 0 100 5 10
    240613114142_zezv CTGTCTGGGAGATCGAGCGACTGTAGGGAAAG(+AGGACA)GATCCAGGCAGTTGCTCCCGGGAATTTTTTT NGG 10 8 17 8 24 0 100 5 10
    240613114141_k9tl CCTTGGTTCTGTTGCTTACTGGCCGAGAAACG(+AGGACA)GTACCAATCTTTCAGCTCTGTGCTTGTCGGC NGG 10 8 17 8 24 0 100 5 10
    240613114140_yf46 AAGAAGCCGACAAGCACAGAGCTGAAAGATTG(+AGGACA)GTACCGTTTCTCGGCCAGTAAGCAACAGAAC NGG 10 8 17 8 24 0 100 5 10
    240613114138_si45 TCGTCAGCAGCCTGTCTCATTGCTTCAGCTGG(+AGGACA)GTACCAGTCCTTCTGTTCCCCTGGGAAACTG NGG 10 8 17 8 24 0 100 5 10