Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613114250_x8qf CACAGTGGTGTGATTGTACTAACCACTAGGAG(+AGGACA)GATCCCATATGCCTACCTGTTGCCATGCCAA NGG 10 8 17 8 24 0 100 5 10
    240613114249_n1ah ACATGGACCTTTGGAAACCTGTGGATGACATG(+AGGACA)GATCCGTGAAGAGCCCGGGTCACTACCACTC NGG 10 8 17 8 24 0 100 5 10
    240613114248_so12 GAAATGAGTGGTAGTGACCCGGGCTCTTCACG(+AGGACA)GATCCATGTCATCCACAGGTTTCCAAAGGTC NGG 10 8 17 8 24 0 100 5 10
    240613114247_w5a9 TCAAATCTATACACAGTAGGGCTATGAAGTTG(+AGGACA)GTACCTCAGAAGTTCCTCCCCTGCCTGTTGG NGG 10 8 17 8 24 0 100 5 10
    240613114246_owrt AGGTCCCAACAGGCAGGGGAGGAACTTCTGAG(+AGGACA)GTACCAACTTCATAGCCCTACTGTGTATAGA NGG 10 8 17 8 24 0 100 5 10
    240613114244_j3rj GCTCCGAGGTCAGGTGGACTGTCAGGGCCTAG(+AGGACA)GATCCACCAGTTTGCCCGAGTTTACATTAGC NGG 10 8 17 8 24 0 100 5 10
    240613114243_l1yx ATGTTGCTAATGTAAACTCGGGCAAACTGGTG(+AGGACA)GATCCTAGGCCCTGACAGTCCACCTGACCTC NGG 10 8 17 8 24 0 100 5 10
    240613114240_cm8s CTCCTGAGCCAATAGGGGCTTCCAGACCCAAG(+AGGACA)GATCCGTTTCCTTGATGACTAAGAAGCCCCA NGG 10 8 17 8 24 0 100 5 10
    240613114239_gylx AAGGGTGGGGCTTCTTAGTCATCAAGGAAACG(+AGGACA)GATCCTTGGGTCTGGAAGCCCCTATTGGCTC NGG 10 8 17 8 24 0 100 5 10
    240613114237_p3uc TCCAAGAGCCCTGCTCTGCCCCTCGTGGCATG(+AGGACA)GATCCTTTCCCTGGTGTGGACTTAAGTCTCT NGG 10 8 17 8 24 0 100 5 10