Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613114120_fr01 CTCTTTTCCTTTGAATGTTTTTGCTTCTCTGG(+AGGACA)GTACCTCCTGCACCCCCAACGCTGGCCCTGG NGG 10 8 17 8 24 0 100 5 10
    240613114118_s009 TAAAATTCAGTATTTAAATATTAATTGCCAGG(+AGGACA)GTACCAAATAAATTGTCACTTATAAATATTT NGG 10 8 17 8 24 0 100 5 10
    240613114117_z7nx ACAAGAAATATTTATAAGTGACAATTTATTTG(+AGGACA)GTACCCTGGCAATTAATATTTAAATACTGAA NGG 10 8 17 8 24 0 100 5 10
    240613114116_kzmd TGTGAAAACAGCCCTTTTAGAGTATTTATTAG(+AGGACA)GATCCTAAGCTAATCGTAGCCTTCACAAATT NGG 10 8 17 8 24 0 100 5 10
    240613114115_xt2o GTAACAATTTGTGAAGGCTACGATTAGCTTAG(+AGGACA)GATCCTAATAAATACTCTAAAAGGGCTGTTT NGG 10 8 17 8 24 0 100 5 10
    240613114113_ofq1 GGATTGCCCCACACCGTCCCCAGTCAGCCCTG(+AGGACA)GATCCATGTGTCCTTTACTTGGGGAAAACCT NGG 10 8 17 8 24 0 100 5 10
    240613114111_knif GTAGAAGGTTTTCCCCAAGTAAAGGACACATG(+AGGACA)GATCCAGGGCTGACTGGGGACGGTGTGGGGC NGG 10 8 17 8 24 0 100 5 10
    240613114110_ksel TGGCCTGCTACAAACCTAGGGAGGGAGAGAGG(+AGGACA)GATCCGGGCAAGGTTCGTATTGTGTCCCTGG NGG 10 8 17 8 24 0 100 5 10
    240613114107_gkps CCCAGCCAGGGACACAATACGAACCTTGCCCG(+AGGACA)GATCCCTCTCTCCCTCCCTAGGTTTGTAGCA NGG 10 8 17 8 24 0 100 5 10
    240613114105_efpx CAGCTGACAGTGGTGCACCTTTCACAGCCAGG(+AGGACA)GATCCATGGGGCAGGGGACAGGTGCAGGCGC NGG 10 8 17 8 24 0 100 5 10