Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613114022_7iyg AACACTCATCGTCCTGGACATGTGGGGGGCAG(+AGGACA)GATCCTGGTTTATGTTTTACACTTCTAAATA NGG 10 8 17 8 24 0 100 5 10
    240613114020_qqpe TAAATTTCACCCTCTGCATTACTCACAGAGTG(+AGGACA)GATCCATTGGAGTTTCAGCTGTATGCTAGTT NGG 10 8 17 8 24 0 100 5 10
    240613114018_hhym AGCTGAACTAGCATACAGCTGAAACTCCAATG(+AGGACA)GATCCACTCTGTGAGTAATGCAGAGGGTGAA NGG 10 8 17 8 24 0 100 5 10
    240613114017_ac5n GCCAACATTCCTAAGACTGAACCAACATCCAG(+AGGACA)GTACCCATTTTCTCAGGTTTCCCAGAGATAT NGG 10 8 17 8 24 0 100 5 10
    240613114015_d775 GCCACATATCTCTGGGAAACCTGAGAAAATGG(+AGGACA)GTACCTGGATGTTGGTTCAGTCTTAGGAATG NGG 10 8 17 8 24 0 100 5 10
    240613114014_cxjk AGAGAAGAACTCTTATAGACTTGGGGAGAGAG(+AGGACA)GTACCAACCTTGACATTGACTGTACTGGTGA NGG 10 8 17 8 24 0 100 5 10
    240613114013_zt2z AATACTCACCAGTACAGTCAATGTCAAGGTTG(+AGGACA)GTACCTCTCTCCCCAAGTCTATAAGAGTTCT NGG 10 8 17 8 24 0 100 5 10
    240613114011_n33z TGGAACCCAGTTACAGAAATTGTGTGATATAG(+AGGACA)GTACCTTTTTCTCTTTCTCTGTCCAAGACAT NGG 10 8 17 8 24 0 100 5 10
    240613114010_yky7 AAAGCATGTCTTGGACAGAGAAAGAGAAAAAG(+AGGACA)GTACCTATATCACACAATTTCTGTAACTGGG NGG 10 8 17 8 24 0 100 5 10
    240613114009_6x2e CCATTTCAAGGTGCAGTGTGAAGTATGACTAG(+AGGACA)GTACCTCTCCATTTATTGAGGCTGGAACAGG NGG 10 8 17 8 24 0 100 5 10