Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613114007_m6u9 GATTTCCTGTTCCAGCCTCAATAAATGGAGAG(+AGGACA)GTACCTAGTCATACTTCACACTGCACCTTGA NGG 10 8 17 8 24 0 100 5 10
    240613114006_85gf CTTCAGCTGGAGTGTGCTCTGCTGGTGCAGTG(+AGGACA)GATCCTAGTGTGGTTCCCAGTACTCAACCTG NGG 10 8 17 8 24 0 100 5 10
    240613114005_1zk0 CCAACCAGGTTGAGTACTGGGAACCACACTAG(+AGGACA)GATCCACTGCACCAGCAGAGCACACTCCAGC NGG 10 8 17 8 24 0 100 5 10
    240613114004_fbmj AGAGACAAAGGACACAGGAACCCGGCTGACTG(+AGGACA)GTACCAAAGTGCACTCTCTTCAGGTGCCTCT NGG 10 8 17 8 24 0 100 5 10
    240613114003_7ouh TGTGTAGAGGCACCTGAAGAGAGTGCACTTTG(+AGGACA)GTACCAGTCAGCCGGGTTCCTGTGTCCTTTG NGG 10 8 17 8 24 0 100 5 10
    240613114001_fh7q TGTCTTTATTCTAATGTCTTTGGTGTGGGAAG(+AGGACA)GTACCTCATAGGCTGCCAAAAGAGAAATGCA NGG 10 8 17 8 24 0 100 5 10
    240613114000_7ksh ATGTTTGCATTTCTCTTTTGGCAGCCTATGAG(+AGGACA)GTACCTTCCCACACCAAAGACATTAGAATAA NGG 10 8 17 8 24 0 100 5 10
    240613113959_vgvh TGGGTATGGATGGAGCCACACTCCTATGCAAG(+AGGACA)GATCCATTTCCATGCAAGATTGTCAATTCTT NGG 10 8 17 8 24 0 100 5 10
    240613113957_gdw4 TCTGGAAGAATTGACAATCTTGCATGGAAATG(+AGGACA)GATCCTTGCATAGGAGTGTGGCTCCATCCAT NGG 10 8 17 8 24 0 100 5 10
    240613113956_snn3 TTTTTAAGATGCTTCTGGAGATTTAGAATCAG(+AGGACA)GATCCAAAATAAGGCTGGGATTCAGCATCCA NGG 10 8 17 8 24 0 100 5 10