Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613113808_uf8n AAAGTTCTCATTCCTGCCAGGGTGCCCCAGAG(+AGGACA)GTACCCAAGAGAGCAGTGTAGGGACAAGGAA NGG 10 8 17 8 24 0 100 5 10
    240613113807_t4te GTGGTACAGGGTCCTGGTCTGCTGTGTGTGGG(+AGGACA)GATCCTGGCTGCAGAGCTTCTTCTGAGTAGA NGG 10 8 17 8 24 0 100 5 10
    240613113805_r23q TACCTTCTACTCAGAAGAAGCTCTGCAGCCAG(+AGGACA)GATCCCCACACACAGCAGACCAGGACCCTGT NGG 10 8 17 8 24 0 100 5 10
    240613113804_vo1m CAGCAGTTTTAAAGTCTAACACATTTTAAACG(+AGGACA)GATCCAAAGTCTTCTTTCCTCATCCGTCACT NGG 10 8 17 8 24 0 100 5 10
    240613113802_lbcl TGCTAAGTGACGGATGAGGAAAGAAGACTTTG(+AGGACA)GATCCGTTTAAAATGTGTTAGACTTTAAAAC NGG 10 8 17 8 24 0 100 5 10
    240613113801_b1ge GGCACTGGAGGAAACTATTTCTCCCTCTCAAG(+AGGACA)GATCCGGGCGTGCTCTGCACCCGGCCTTGGT NGG 10 8 17 8 24 0 100 5 10
    240613113759_vqgb AGGCCACCAAGGCCGGGTGCAGAGCACGCCCG(+AGGACA)GATCCTTGAGAGGGAGAAATAGTTTCCTCCA NGG 10 8 17 8 24 0 100 5 10
    240613113757_viqb TGACAGGCTCTGGAGCTGCCTCCTAGGCTGGG(+AGGACA)GTACCCCTAGGCCCTGGCAACCCACGTTGTT NGG 10 8 17 8 24 0 100 5 10
    240613113755_eiii TGTAAAACAACGTGGGTTGCCAGGGCCTAGGG(+AGGACA)GTACCCCAGCCTAGGAGGCAGCTCCAGAGCC NGG 10 8 17 8 24 0 100 5 10
    240613113753_g28u TGGTCCTATTCTAGAGAAGAAAGCCTTGCTGG(+AGGACA)GTACCAATTATGGACTATGGGAGGTAGGATG NGG 10 8 17 8 24 0 100 5 10