Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613113704_bz8h ACACCCAATACCCAGTGTCAATGATGCATGGG(+AGGACA)GATCCAACCAGATCCTGTGCATGTAAGACAA NGG 10 8 17 8 24 0 100 5 10
    240613113703_a5nc CAGCAGGCAGAGGTGAAAAGAACACTGCTGTG(+AGGACA)GATCCCAATCAAAAGTGTTTTACATGCAAAG NGG 10 8 17 8 24 0 100 5 10
    240613113702_sxw0 TATGGCTTTGCATGTAAAACACTTTTGATTGG(+AGGACA)GATCCACAGCAGTGTTCTTTTCACCTCTGCC NGG 10 8 17 8 24 0 100 5 10
    240613113700_8yj6 CCAGGCCAGCACAGTGCTTCCTGGTAGGGTGG(+AGGACA)GTACCAGCCATACTGAGAATCGGGCCAGGCC NGG 10 8 17 8 24 0 100 5 10
    240613113658_6d8y GAGCAGGCCTGGCCCGATTCTCAGTATGGCTG(+AGGACA)GTACCCACCCTACCAGGAAGCACTGTGCTGG NGG 10 8 17 8 24 0 100 5 10
    240613113657_iw7b TCTCTTCAGGGGCCGCTTTCCTGGTGTCAGAG(+AGGACA)GTACCCATGGTGGAGCAGAGCCACCTTCCTC NGG 10 8 17 8 24 0 100 5 10
    240613113655_f1x7 TCTGGGAGGAAGGTGGCTCTGCTCCACCATGG(+AGGACA)GTACCTCTGACACCAGGAAAGCGGCCCCTGA NGG 10 8 17 8 24 0 100 5 10
    240613113654_2y6l TCTCTAGCACCTGGCTCCTGTGGCTCTCTATG(+AGGACA)GTACCTGGAATCCCCAAGGTGTTTAACTCCT NGG 10 8 17 8 24 0 100 5 10
    240613113652_c1as GAGAAAGGAGTTAAACACCTTGGGGATTCCAG(+AGGACA)GTACCATAGAGAGCCACAGGAGCCAGGTGCT NGG 10 8 17 8 24 0 100 5 10
    240613113649_pbs7 ATCTTACAGTCAGCAATCTGGGGGTGGGGAGG(+AGGACA)GATCCCTCTCTAGCACCTGGCTCCTGTGGCT NGG 10 8 17 8 24 0 100 5 10