Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613113648_czeg TAGAGAGCCACAGGAGCCAGGTGCTAGAGAGG(+AGGACA)GATCCCTCCCCACCCCCAGATTGCTGACTGT NGG 10 8 17 8 24 0 100 5 10
    240613113646_xnfp GGCAAAGACTGAGCTCAGGCACCAGCCTCCCG(+AGGACA)GTACCACGCTGGAGGTGGATCCCTGAGTAGA NGG 10 8 17 8 24 0 100 5 10
    240613113645_aqm9 TGCCTTCTACTCAGGGATCCACCTCCAGCGTG(+AGGACA)GTACCGGGAGGCTGGTGCCTGAGCTCAGTCT NGG 10 8 17 8 24 0 100 5 10
    240613113643_mnos GGCACCAGCCTCCCGGTACCACGCTGGAGGTG(+AGGACA)GATCCCTGAGTAGAAGGCAGGGTAAGCTTCG NGG 10 8 17 8 24 0 100 5 10
    240613113642_l2yg GAAGTCGAAGCTTACCCTGCCTTCTACTCAGG(+AGGACA)GATCCACCTCCAGCGTGGTACCGGGAGGCTG NGG 10 8 17 8 24 0 100 5 10
    240613113640_y91s CTGTGCCAGAGCCGCTAGCAGCTGCTCACAGG(+AGGACA)GTACCTTTGTGGGTCTGGTGTTTTGAGAAAT NGG 10 8 17 8 24 0 100 5 10
    240613113639_7w5c GGGTGATTTCTCAAAACACCAGACCCACAAAG(+AGGACA)GTACCCTGTGAGCAGCTGCTAGCGGCTCTGG NGG 10 8 17 8 24 0 100 5 10
    240613113637_zi56 GAGACGAGGGGCGGGAGGTCAGCTTCTCCCTG(+AGGACA)GATCCTCAGTGTGCACTGCCTTGGGCAGGGA NGG 10 8 17 8 24 0 100 5 10
    240613113635_xzf3 GTGGCTCCCTGCCCAAGGCAGTGCACACTGAG(+AGGACA)GATCCAGGGAGAAGCTGACCTCCCGCCCCTC NGG 10 8 17 8 24 0 100 5 10
    240613113634_nd5f GACCCGCGGCTTGCGCATCTGTGACATTTCTG(+AGGACA)GATCCCGGTCAGTTCCGGCTGTGCCCAGACT NGG 10 8 17 8 24 0 100 5 10