Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613113546_35gm GCGCCGTGCTTTAGCCTTCTGGAAATCAGTTG(+AGGACA)GTACCTCCCCACTCCAGAGGAAGTCAGTTGA NGG 10 8 17 8 24 0 100 5 10
    240613113544_utom TAGAAGCGGGGCTGCCTGCCCAGGAGGACTGG(+AGGACA)GATCCTCTCTGCTCAGGGAAGCACAGGCCAG NGG 10 8 17 8 24 0 100 5 10
    240613113543_jwe5 GAGGCCTGGCCTGTGCTTCCCTGAGCAGAGAG(+AGGACA)GATCCCAGTCCTCCTGGGCAGGCAGCCCCGC NGG 10 8 17 8 24 0 100 5 10
    240613113542_6rqj AGGCAAGGGAGGAGCAATCATTTTCCGCAATG(+AGGACA)GTACCAATGGATGGGGAGGTACTGGTGAGGG NGG 10 8 17 8 24 0 100 5 10
    240613113540_8og1 CCCACCCCTCACCAGTACCTCCCCATCCATTG(+AGGACA)GTACCATTGCGGAAAATGATTGCTCCTCCCT NGG 10 8 17 8 24 0 100 5 10
    240613113538_ghxf AGTGCAATCTGAAATGACCTGGACAAGGAATG(+AGGACA)GTACCCCATTGGGTTGAAAAATTCACATAGC NGG 10 8 17 8 24 0 100 5 10
    240613113537_v2g6 TCCTGGCTATGTGAATTTTTCAACCCAATGGG(+AGGACA)GTACCATTCCTTGTCCAGGTCATTTCAGATT NGG 10 8 17 8 24 0 100 5 10
    240613113536_6gva CCACAGACCCATTGGGGCCAGGTCTAGCTCAG(+AGGACA)GTACCTGAGCAAATGTTCCTTTAGAGTCCAC NGG 10 8 17 8 24 0 100 5 10
    240613113534_aq1p CCCTGGTGGACTCTAAAGGAACATTTGCTCAG(+AGGACA)GTACCTGAGCTAGACCTGGCCCCAATGGGTC NGG 10 8 17 8 24 0 100 5 10
    240613113533_9ydu ACTTGTTAAGAGGCAAGGCCACCTTTCACCAG(+AGGACA)GTACCCTGGCCAGGCACCTGGGAAGACTCTT NGG 10 8 17 8 24 0 100 5 10