Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613113515_kdew TAGTCCCCTGTCCTCAGAATGGGATTCTCACG(+AGGACA)GTACCCTTTGTCAGCCACTCTGTTGCCCCCA NGG 10 8 17 8 24 0 100 5 10
    240613113514_crid CAGGTGCATAGCTGGTCTTCCCAGTTGGTGAG(+AGGACA)GATCCAGACCCTGTGCAGTTTGATGCTCAGT NGG 10 8 17 8 24 0 100 5 10
    240613113513_ahly ACGTGACTGAGCATCAAACTGCACAGGGTCTG(+AGGACA)GATCCTCACCAACTGGGAAGACCAGCTATGC NGG 10 8 17 8 24 0 100 5 10
    240613113511_o43a TTCACCCTTAGAAAGGGTTGGGGCTACTCTGG(+AGGACA)GATCCTGAACACCCAGCATAGTCCCTGGATG NGG 10 8 17 8 24 0 100 5 10
    240613113510_b96l TTAGCCATCCAGGGACTATGCTGGGTGTTCAG(+AGGACA)GATCCCAGAGTAGCCCCAACCCTTTCTAAGG NGG 10 8 17 8 24 0 100 5 10
    240613113509_kcjj GAAAGCACAAATCCTAAGCACAGCCTTTGAAG(+AGGACA)GATCCTGCTAATTGTATGTCTTAACAATAAA NGG 10 8 17 8 24 0 100 5 10
    240613113508_vqzg AAGATTTTATTGTTAAGACATACAATTAGCAG(+AGGACA)GATCCTTCAAAGGCTGTGCTTAGGATTTGTG NGG 10 8 17 8 24 0 100 5 10
    240613113507_hcz3 ACAAGTACAGTTTCCAGTGCCTCCCTGCTGTG(+AGGACA)GTACCTGAAAAGTCTGAATGCAAGTAGTATG NGG 10 8 17 8 24 0 100 5 10
    240613113505_1tum ATAGTCATACTACTTGCATTCAGACTTTTCAG(+AGGACA)GTACCACAGCAGGGAGGCACTGGAAACTGTA NGG 10 8 17 8 24 0 100 5 10
    240613113503_ic47 AAAAATGTTCCTGCAAAGGACATCCACTCCTG(+AGGACA)GATCCTGATGCCATTCACACAATGAGATAGT NGG 10 8 17 8 24 0 100 5 10