Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613113431_t98l TCAAGAAGCCAGCGCAAGAGATTTCAGCCTGG(+AGGACA)GTACCAGCAACTTCCAGTTCTAAGTCAGCCA NGG 10 8 17 8 24 0 100 5 10
    240613113320_ejng TGTGGATAACATATTATGTATATATGTAATGG(+AGGACA)GTACCAGGCCAGGTTTATATTTTTTTCTTTA NGG 10 8 17 8 24 0 100 5 10
    240613113315_bm11 ACGTCTAAAGAAAAAAATATAAACCTGGCCTG(+AGGACA)GTACCCATTACATATATACATAATATGTTAT NGG 10 8 17 8 24 0 100 5 10
    240613113305_4j3m GCTCACAACCATGCATAACTCAAGTTTTAAGG(+AGGACA)GATCCAGTGCTGACTTCTGGCCTCTGAGGGT NGG 10 8 17 8 24 0 100 5 10
    240613113304_vf1f GCAGTACCCTCAGAGGCCAGAAGTCAGCACTG(+AGGACA)GATCCCTTAAAACTTGAGTTATGCATGGTTG NGG 10 8 17 8 24 0 100 5 10
    240613113303_gjs0 TTGGGGGAGATGCAGCACACACCCCAGATAGG(+AGGACA)GATCCTATGACTGACCAACAAAAGTACAGGT NGG 10 8 17 8 24 0 100 5 10
    240613113301_awqs GTGGTACCTGTACTTTTGTTGGTCAGTCATAG(+AGGACA)GATCCCTATCTGGGGTGTGTGCTGCATCTCC NGG 10 8 17 8 24 0 100 5 10
    240613113300_y9aw CATGCCATTTTAGGGCCATTGTGATTCTCAGG(+AGGACA)GATCCTAGGTAAAAATCCTGAATCTTTTGGG NGG 10 8 17 8 24 0 100 5 10
    240613113258_h5zw TCTTACCCAAAAGATTCAGGATTTTTACCTAG(+AGGACA)GATCCCTGAGAATCACAATGGCCCTAAAATG NGG 10 8 17 8 24 0 100 5 10
    240613113256_dtmj TCTTGCTTGTCAACTTCAGAGATAGCTGGAAG(+AGGACA)GATCCTGAGACTGTATGGCAGGAGAGTTGAG NGG 10 8 17 8 24 0 100 5 10