Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613113255_2zux AACCCCTCAACTCTCCTGCCATACAGTCTCAG(+AGGACA)GATCCTTCCAGCTATCTCTGAAGTTGACAAG NGG 10 8 17 8 24 0 100 5 10
    240613113254_vwfm AAAAGCATGAGATTTGCATCCCAGCTGTCCTG(+AGGACA)GATCCAGGCTGTGAGGTTTTGAGGATTCTTA NGG 10 8 17 8 24 0 100 5 10
    240613113252_ehx2 GTGAGTAAGAATCCTCAAAACCTCACAGCCTG(+AGGACA)GATCCAGGACAGCTGGGATGCAAATCTCATG NGG 10 8 17 8 24 0 100 5 10
    240613113250_u52d TGGTTCATCTGTTTGAGAGCCTTTGGGAAGGG(+AGGACA)GATCCTGTTTGCTGGAAAATGTTGGGAGATC NGG 10 8 17 8 24 0 100 5 10
    240613113249_pw1h TCTGGGATCTCCCAACATTTTCCAGCAAACAG(+AGGACA)GATCCCCTTCCCAAAGGCTCTCAAACAGATG NGG 10 8 17 8 24 0 100 5 10
    240613113248_6svh TAATTCTGGAACCCCAGGGAAGCCAAGATCAG(+AGGACA)GATCCTGTTGATCTCTTGTTTTCTAAGGCCT NGG 10 8 17 8 24 0 100 5 10
    240613113247_b0j0 GAAGAAGGCCTTAGAAAACAAGAGATCAACAG(+AGGACA)GATCCTGATCTTGGCTTCCCTGGGGTTCCAG NGG 10 8 17 8 24 0 100 5 10
    240613113246_urq8 TACTTTGTCAGAGTTCTGACTGTCAGTCACAG(+AGGACA)GATCCTATTAGTGTTGCAAGTCTAGCAAGTA NGG 10 8 17 8 24 0 100 5 10
    240613113244_pw5g CACTCTACTTGCTAGACTTGCAACACTAATAG(+AGGACA)GATCCTGTGACTGACAGTCAGAACTCTGACA NGG 10 8 17 8 24 0 100 5 10
    240613113243_zdxk GTAATCCCCTCGTTGGGAGGTGGAGACAAGAG(+AGGACA)GATCCCTGAGGCTTGCTGGGCTGCTAATATA NGG 10 8 17 8 24 0 100 5 10