Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613113823_kccx AGCAGTGGAAATCATAGTCCTTGGCTGGCATG(+AGGACA)GATCCAACCCCAGGCTTGTGATTGGGTGGTG NGG 10 8 17 8 24 0 100 5 10
    240613113822_wcbz GCTTGGCTACCTGTAGACTCTAATCCTAAGTG(+AGGACA)GATCCAGAATTGCTTTCCCAAGTCTAACTTC NGG 10 8 17 8 24 0 100 5 10
    240613113821_pab3 GAACTGAAGTTAGACTTGGGAAAGCAATTCTG(+AGGACA)GATCCACTTAGGATTAGAGTCTACAGGTAGC NGG 10 8 17 8 24 0 100 5 10
    240613113819_7wny AACCTAGAAACTGTAGGTGGGCATATATCAGG(+AGGACA)GTACCTATGCCACCGCCAACTTCTCCTTTGT NGG 10 8 17 8 24 0 100 5 10
    240613113818_y0mb CTTGGACAAAGGAGAAGTTGGCGGTGGCATAG(+AGGACA)GTACCCTGATATATGCCCACCTACAGTTTCT NGG 10 8 17 8 24 0 100 5 10
    240613113816_c5jg AATGTACTAAATGATTCTCCTCCTCTCTCCTG(+AGGACA)GTACCTGGAGACCCCTGGCTATCATGGGATT NGG 10 8 17 8 24 0 100 5 10
    240613113814_73ba TCAGTAATCCCATGATAGCCAGGGGTCTCCAG(+AGGACA)GTACCAGGAGAGAGGAGGAGAATCATTTAGT NGG 10 8 17 8 24 0 100 5 10
    240613113813_231m ACTCAGAAAAGAACCATAGGCACACACGTGTG(+AGGACA)GATCCTATATCTTGATATTATATAGATACAC NGG 10 8 17 8 24 0 100 5 10
    240613113812_9i01 GGATGGTGTATCTATATAATATCAAGATATAG(+AGGACA)GATCCACACGTGTGTGCCTATGGTTCTTTTC NGG 10 8 17 8 24 0 100 5 10
    240613113811_r5cq GGCTATTCCTTGTCCCTACACTGCTCTCTTGG(+AGGACA)GTACCTCTGGGGCACCCTGGCAGGAATGAGA NGG 10 8 17 8 24 0 100 5 10