Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613114049_2l93 CTAGAATTTGAATATGTGGTAGAGCCTCAAAG(+AGGACA)GATCCTTTGTGATCAGGTAAATACTACCTTG NGG 10 8 17 8 24 0 100 5 10
    240613114048_ym4z GGCTTAGTTTTTATTTAATAAGATATTTAATG(+AGGACA)GATCCATTTTTATCAAGGTCAGAAAACATTT NGG 10 8 17 8 24 0 100 5 10
    240613114047_ytjp ATTTAAAATGTTTTCTGACCTTGATAAAAATG(+AGGACA)GATCCATTAAATATCTTATTAAATAAAAACT NGG 10 8 17 8 24 0 100 5 10
    240613114046_9pz7 AGGACAAAGGCTGGAAACTTCAGATGAACTTG(+AGGACA)GTACCCACTGAGACTTCCCTGCTGAAAGACA NGG 10 8 17 8 24 0 100 5 10
    240613114044_xqkw ACCACTGTCTTTCAGCAGGGAAGTCTCAGTGG(+AGGACA)GTACCAAGTTCATCTGAAGTTTCCAGCCTTT NGG 10 8 17 8 24 0 100 5 10
    240613114042_gb35 AAGAAGCCCGGCGGAGCTTTAGGTGGCCACAG(+AGGACA)GTACCTGCTGGCAGTGCTGTCACATTTAAGG NGG 10 8 17 8 24 0 100 5 10
    240613114041_1mcv CCTTGCCTTAAATGTGACAGCACTGCCAGCAG(+AGGACA)GTACCTGTGGCCACCTAAAGCTCCGCCGGGC NGG 10 8 17 8 24 0 100 5 10
    240613114039_kk46 ACTTGGCTGAGGAGACTCGGCTTAGGAAGCAG(+AGGACA)GTACCTGACTTTTGAGAAAACCCACTAGCTT NGG 10 8 17 8 24 0 100 5 10
    240613114038_yv72 AACAGAAGCTAGTGGGTTTTCTCAAAAGTCAG(+AGGACA)GTACCTGCTTCCTAAGCCGAGTCTCCTCAGC NGG 10 8 17 8 24 0 100 5 10
    240613114037_mixu AGGAATCGATTCATAGTCCTGGGTATCAAAGG(+AGGACA)GATCCAGTCACCAGGAGCTCCTCTCTTACCA NGG 10 8 17 8 24 0 100 5 10