Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613114610_1pde GCTTTACTCGGCCACTGAAGACTCGGCCACAG(+AGGACA)GATCCTCCCTGGACCCTCACAGCTGGGAGGG NGG 10 8 17 8 24 0 100 5 10
    240613114609_z704 TGTCAGGAAAAACACATGCCCATGAAGGAATG(+AGGACA)GTACCCTCTAGTACCACCAAGCTCCACATGA NGG 10 8 17 8 24 0 100 5 10
    240613114607_qjz7 ATGGCTCATGTGGAGCTTGGTGGTACTAGAGG(+AGGACA)GTACCATTCCTTCATGGGCATGTGTTTTTCC NGG 10 8 17 8 24 0 100 5 10
    240613114605_au4n ACTGAAATGGGAATGTTCCCAAGGGGCCTGGG(+AGGACA)GATCCCCTGTGAGGAGAAGTCCTTGAGCCTG NGG 10 8 17 8 24 0 100 5 10
    240613114603_kk0o AGCTTCAGGCTCAAGGACTTCTCCTCACAGGG(+AGGACA)GATCCCCAGGCCCCTTGGGAACATTCCCATT NGG 10 8 17 8 24 0 100 5 10
    240613114601_bdg7 GGTGGATATCCTGTAACATAAATGCTCGTTTG(+AGGACA)GATCCAAAAGGAAAACCATTAAAAATAAAAG NGG 10 8 17 8 24 0 100 5 10
    240613114600_zygo TCTTCCTTTTATTTTTAATGGTTTTCCTTTTG(+AGGACA)GATCCAAACGAGCATTTATGTTACAGGATAT NGG 10 8 17 8 24 0 100 5 10
    240613114558_l3e6 TAACGTTGACACAAACACAGATAAGCTCGTGG(+AGGACA)GTACCTGGACAAAAGGGTGCCTTAGTTTATG NGG 10 8 17 8 24 0 100 5 10
    240613114557_zhx8 GAGGACATAAACTAAGGCACCCTTTTGTCCAG(+AGGACA)GTACCCACGAGCTTATCTGTGTTTGTGTCAA NGG 10 8 17 8 24 0 100 5 10
    240613114555_5k7c GTGACAAGTTAGAATGTCTCTCTTGGTTTGTG(+AGGACA)GTACCCCCAGGTTGGCTTTGTTCTGTGGAAA NGG 10 8 17 8 24 0 100 5 10