Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613114714_yioq ATGACCAGTCTTCTCCCCTTCTCTGCCAATTG(+AGGACA)GTACCCACCAGATGCTCTTCCCACTTCTCTC NGG 10 8 17 8 24 0 100 5 10
    240613114712_ohu6 TCTCTTCCACTCAGAAAGTGGCATGGCTTCAG(+AGGACA)GTACCACAGGGCACATATGTCAATCAACCCT NGG 10 8 17 8 24 0 100 5 10
    240613114711_qxjj AGACCAGGGTTGATTGACATATGTGCCCTGTG(+AGGACA)GTACCTGAAGCCATGCCACTTTCTGAGTGGA NGG 10 8 17 8 24 0 100 5 10
    240613114709_bik3 CCCATGAGAGTGTCGGGGTCAGGGGCTGAAAG(+AGGACA)GATCCAACATTTCCAAGTTAGAAGTTAAGCT NGG 10 8 17 8 24 0 100 5 10
    240613114708_h8ia GTTAAAGCTTAACTTCTAACTTGGAAATGTTG(+AGGACA)GATCCTTTCAGCCCCTGACCCCGACACTCTC NGG 10 8 17 8 24 0 100 5 10
    240613114707_548j TGGCAGGATCAACATGTGTAACTGCCTTCTTG(+AGGACA)GTACCTGTTTGATTAGTGGGTAGAAAATAGC NGG 10 8 17 8 24 0 100 5 10
    240613114706_u9ma TAACTGCTATTTTCTACCCACTAATCAAACAG(+AGGACA)GTACCAAGAAGGCAGTTACACATGTTGATCC NGG 10 8 17 8 24 0 100 5 10
    240613114704_98vq AAGAGGTCACACGGTTTGGAAACTTGGGGTAG(+AGGACA)GTACCAGAGCATACAAGTTTAAATCAGGGCA NGG 10 8 17 8 24 0 100 5 10
    240613114703_gho7 TGCAATGCCCTGATTTAAACTTGTATGCTCTG(+AGGACA)GTACCTACCCCAAGTTTCCAAACCGTGTGAC NGG 10 8 17 8 24 0 100 5 10
    240613114701_tl0a CTTACTTAGGTATCAGTAACCTTCAGCTGAGG(+AGGACA)GATCCACTGGGTAGTGGATTGTGCTATCTGT NGG 10 8 17 8 24 0 100 5 10