Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613112908_o1ca ACTGTCTTTACACACACTAGAAGAGGGCATTG(+AGGACA)GATCCCATTACATGTGGTTGCTGGGAATTGA NGG 10 8 17 8 24 0 100 5 10
    240613112907_l74w TGAGTTCAATTCCCAGCAACCACATGTAATGG(+AGGACA)GATCCAATGCCCTCTTCTAGTGTGTGTAAAG NGG 10 8 17 8 24 0 100 5 10
    240613112905_iud2 TGACCCGTGCTTTGAGAACTCGGGAAAACCAG(+AGGACA)GATCCATGTCCACTTCACTTCGACTTTATCT NGG 10 8 17 8 24 0 100 5 10
    240613112904_m8ba TGAGGAGATAAAGTCGAAGTGAAGTGGACATG(+AGGACA)GATCCTGGTTTTCCCGAGTTCTCAAAGCACG NGG 10 8 17 8 24 0 100 5 10
    240613112902_bae5 CATGTAACGGAAGGGTGGGCGCAGATCCTTAG(+AGGACA)GTACCAGGGTGCTGTGCAATGGAAGATGCAG NGG 10 8 17 8 24 0 100 5 10
    240613112859_4n1s CATCTCTGCATCTTCCATTGCACAGCACCCTG(+AGGACA)GTACCTAAGGATCTGCGCCCACCCTTCCGTT NGG 10 8 17 8 24 0 100 5 10
    240613112858_scby ACACACACACACACAAATACTCAAGAGGTGGG(+AGGACA)GATCCACAAATATGAAGTAAACCAGAGCAGT NGG 10 8 17 8 24 0 100 5 10
    240613112856_15qb AATCAACTGCTCTGGTTTACTTCATATTTGTG(+AGGACA)GATCCCCACCTCTTGAGTATTTGTGTGTGTG NGG 10 8 17 8 24 0 100 5 10
    240613112855_55xu AGTAAGAGTTCCATTTTCTCACATCAGTGAAG(+AGGACA)GTACCATACATTGAAACTCTCAGTACTATAT NGG 10 8 17 8 24 0 100 5 10
    240613112854_7957 TAGAAATATAGTACTGAGAGTTTCAATGTATG(+AGGACA)GTACCTTCACTGATGTGAGAAAATGGAACTC NGG 10 8 17 8 24 0 100 5 10