Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613113011_mfy3 TTCATTTTAGATTACTAATAAAGGGAGAAAAG(+AGGACA)GTACCTTCAAAGCTTATTCACATTTGCAAAA NGG 10 8 17 8 24 0 100 5 10
    240613113009_x6lb AAAGATTTTGCAAATGTGAATAAGCTTTGAAG(+AGGACA)GTACCTTTTCTCCCTTTATTAGTAATCTAAA NGG 10 8 17 8 24 0 100 5 10
    240613113008_etil TCATTTCCCAGAGGGCGTCAGATGGTGGTATG(+AGGACA)GATCCTGCCTCCTGAACTCAATCCTTTCAGT NGG 10 8 17 8 24 0 100 5 10
    240613113007_4rkx CCTGCACTGAAAGGATTGAGTTCAGGAGGCAG(+AGGACA)GATCCATACCACCATCTGACGCCCTCTGGGA NGG 10 8 17 8 24 0 100 5 10
    240613113005_mcvo AGTCATTGTTAGGTGTTCGTGTTGGGAATGGG(+AGGACA)GATCCTTCAAGGAACAGAGTAAAGCACCTTC NGG 10 8 17 8 24 0 100 5 10
    240613113004_3jqd AGGGTGAAGGTGCTTTACTCTGTTCCTTGAAG(+AGGACA)GATCCCCATTCCCAACACGAACACCTAACAA NGG 10 8 17 8 24 0 100 5 10
    240613113003_8dvu TACACCGGGTCTCTTTAAAATTGGCATGCTCG(+AGGACA)GTACCAAATAGGTGAGCTCATAATTAGAAAT NGG 10 8 17 8 24 0 100 5 10
    240613113002_47w6 CTGTAATTTCTAATTATGAGCTCACCTATTTG(+AGGACA)GTACCGAGCATGCCAATTTTAAAGAGACCCG NGG 10 8 17 8 24 0 100 5 10
    240613113000_2rbd ATCCAGAGCAGTGATGATGAGCAGGTAGACGG(+AGGACA)GTACCAGCCGGCGGGCTTCTGGATAGGGGTC NGG 10 8 17 8 24 0 100 5 10
    240613112958_9c71 ACAGGGACCCCTATCCAGAAGCCCGCCGGCTG(+AGGACA)GTACCCGTCTACCTGCTCATCATCACTGCTC NGG 10 8 17 8 24 0 100 5 10