Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613113055_bed5 CACACACGTGCATGCTCTGTGCGTGTATGTGG(+AGGACA)GTACCGTGTATATGTGTGTGAGTGTTGTATA NGG 10 8 17 8 24 0 100 5 10
    240613113054_cr22 ATGCATATACAACACTCACACACATATACACG(+AGGACA)GTACCCACATACACGCACAGAGCATGCACGT NGG 10 8 17 8 24 0 100 5 10
    240613113052_0zr7 CTACTGATACTTTCAGGGGTGGTCCCATGGGG(+AGGACA)GATCCATGCAGTGGAAGAAACACTGGATTGG NGG 10 8 17 8 24 0 100 5 10
    240613113051_ca4u CGTACCCAATCCAGTGTTTCTTCCACTGCATG(+AGGACA)GATCCCCCATGGGACCACCCCTGAAAGTATC NGG 10 8 17 8 24 0 100 5 10
    240613113049_j00g ATGTAATTAGGCTTTTTGAGGTAGTTCTCAAG(+AGGACA)GATCCTCTTTTGACTCTTGGGAAAGAATTGT NGG 10 8 17 8 24 0 100 5 10
    240613113048_xixl CACGCACAATTCTTTCCCAAGAGTCAAAAGAG(+AGGACA)GATCCTTGAGAACTACCTCAAAAAGCCTAAT NGG 10 8 17 8 24 0 100 5 10
    240613113047_ycii ATGAGGAGTAATCTCCTGTTATTTTCTATTTG(+AGGACA)GTACCAAGATAGCTAATATTACGTGAAATTT NGG 10 8 17 8 24 0 100 5 10
    240613113045_0emf CCAGAAAATTTCACGTAATATTAGCTATCTTG(+AGGACA)GTACCAAATAGAAAATAACAGGAGATTACTC NGG 10 8 17 8 24 0 100 5 10
    240613113044_xufv TTTTCTGGCCCTTTTAAGGGTGCCTACAGTAG(+AGGACA)GATCCAAAGAGCAGCCCAGCACTCGATTTCT NGG 10 8 17 8 24 0 100 5 10
    240613113043_sul3 ATCCTAGAAATCGAGTGCTGGGCTGCTCTTTG(+AGGACA)GATCCTACTGTAGGCACCCTTAAAAGGGCCA NGG 10 8 17 8 24 0 100 5 10