Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613113042_5834 AAAGAGGAAAAAGCCTACAACCCTCTCTGCTG(+AGGACA)GATCCATCCCCACCTCCTCCGGTCAGGGTTC NGG 10 8 17 8 24 0 100 5 10
    240613113041_4t6v TTCTGGAACCCTGACCGGAGGAGGTGGGGATG(+AGGACA)GATCCAGCAGAGAGGGTTGTAGGCTTTTTCC NGG 10 8 17 8 24 0 100 5 10
    240613113039_mxmz CAGATGCCTCCAGCACTGCCATGTGCCCAGTG(+AGGACA)GTACCCCTTGACAGTGTTGTTCTCAGTGACT NGG 10 8 17 8 24 0 100 5 10
    240613113036_8ngh GAACAAGTCACTGAGAACAACACTGTCAAGGG(+AGGACA)GTACCACTGGGCACATGGCAGTGCTGGAGGC NGG 10 8 17 8 24 0 100 5 10
    240613113035_1tvg ATAGACTCGGATCCTTCCTGCATTGTTGCAAG(+AGGACA)GTACCCAATTGTCAGTTGCAGGAGACCCAGA NGG 10 8 17 8 24 0 100 5 10
    240613113034_ghc6 CACACTCTGGGTCTCCTGCAACTGACAATTGG(+AGGACA)GTACCTTGCAACAATGCAGGAAGGATCCGAG NGG 10 8 17 8 24 0 100 5 10
    240613113033_2kf0 TCTGCATAGTTTAAAACCACATGGATCATCTG(+AGGACA)GTACCTTTTTTTCCTAGACAACTTTGATCCT NGG 10 8 17 8 24 0 100 5 10
    240613113031_rjn5 AGACAAGGATCAAAGTTGTCTAGGAAAAAAAG(+AGGACA)GTACCAGATGATCCATGTGGTTTTAAACTAT NGG 10 8 17 8 24 0 100 5 10
    240613113029_b5uv AACCCCCCACAGATGGTGTGTTGAAACCTTGG(+AGGACA)GATCCCAGCATACAGAGTATACTTTTTGTTG NGG 10 8 17 8 24 0 100 5 10
    240613113028_tixm AAATTCAACAAAAAGTATACTCTGTATGCTGG(+AGGACA)GATCCCAAGGTTTCAACACACCATCTGTGGG NGG 10 8 17 8 24 0 100 5 10