Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240613113112_ltm6 GCCTTGTCTAGGAACACAGAAAGGAGATGATG(+AGGACA)GTACCTACTGGCCTTGGACTCTTCTGCGTGA NGG 10 8 17 8 24 0 100 5 10
    240613113110_69j6 GTTTGTCACGCAGAAGAGTCCAAGGCCAGTAG(+AGGACA)GTACCATCATCTCCTTTCTGTGTTCCTAGAC NGG 10 8 17 8 24 0 100 5 10
    240613113109_h20g TGTAAAATTCCAGTGACTCAAATGGCTTTAGG(+AGGACA)GATCCCCTGGAGACGGTTCAGGAAGGGAGTT NGG 10 8 17 8 24 0 100 5 10
    240613113107_u2nc CCAACAACTCCCTTCCTGAACCGTCTCCAGGG(+AGGACA)GATCCCTAAAGCCATTTGAGTCACTGGAATT NGG 10 8 17 8 24 0 100 5 10
    240613113105_50ir GCAAAACTAACCTAAGGCTAAGTGACCACAGG(+AGGACA)GATCCTCTGGGTGAACAGTGAGGACTATAAC NGG 10 8 17 8 24 0 100 5 10
    240613113103_1b5e CTGTGGTTATAGTCCTCACTGTTCACCCAGAG(+AGGACA)GATCCCTGTGGTCACTTAGCCTTAGGTTAGT NGG 10 8 17 8 24 0 100 5 10
    240613113101_rf62 CTCGGTGCATAACCTTGATCGTGGAGCCTCAG(+AGGACA)GATCCCAAAACAGGATGTTTTGGGTGTCAAT NGG 10 8 17 8 24 0 100 5 10
    240613113059_ewfm ATCAGATTGACACCCAAAACATCCTGTTTTGG(+AGGACA)GATCCTGAGGCTCCACGATCAAGGTTATGCA NGG 10 8 17 8 24 0 100 5 10
    240613113058_9e04 TGGAAGAGTTGTGTCCTTCCCCTACGAAGGTG(+AGGACA)GTACCGATGTGCTCTGCCTTTCTGATCCCAT NGG 10 8 17 8 24 0 100 5 10
    240613113057_9mmp ATAAAATGGGATCAGAAAGGCAGAGCACATCG(+AGGACA)GTACCACCTTCGTAGGGGAAGGACACAACTC NGG 10 8 17 8 24 0 100 5 10