Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607035411_zw0h ACATCAGGCACCAGTGAAGATACATCCCCCTG(+ATATAT)GATCCCATTGATGAGGACTCTAAAAGGGGAT NGG 10 8 17 8 24 0 100 5 10
    240607035409_nlpd CTTCTATCCCCTTTTAGAGTCCTCATCAATGG(+ATATAT)GATCCAGGGGGATGTATCTTCACTGGTGCCT NGG 10 8 17 8 24 0 100 5 10
    240607035408_ahq5 AACACAGAGTGGCACACAACCATCTGTAATGG(+ATATAT)GATCCAATGCCTTCTTCTGGTGTGGCTGAAG NGG 10 8 17 8 24 0 100 5 10
    240607035406_lrwc GCTGTCTTCAGCCACACCAGAAGAAGGCATTG(+ATATAT)GATCCCATTACAGATGGTTGTGTGCCACTCT NGG 10 8 17 8 24 0 100 5 10
    240607035405_cygk CAGGCTACCATACACACTCAGCATTTGTGTGG(+ATATAT)GTACCGGGGAACCCTGCTTCTCACTCTTGCA NGG 10 8 17 8 24 0 100 5 10
    240607035402_e98n TGCCATGCAAGAGTGAGAAGCAGGGTTCCCCG(+ATATAT)GTACCCACACAAATGCTGAGTGTGTATGGTA NGG 10 8 17 8 24 0 100 5 10
    240607035400_va5q AATCTTGGACCTGTTTCATGAAGACCCAGTAG(+ATATAT)GTACCCGGATTCTGGCTAGGTTCTCATTCAG NGG 10 8 17 8 24 0 100 5 10
    240607035358_7fqd ACTTGCTGAATGAGAACCTAGCCAGAATCCGG(+ATATAT)GTACCTACTGGGTCTTCATGAAACAGGTCCA NGG 10 8 17 8 24 0 100 5 10
    240607035357_ugcw ACAGTCTGGGTCTTTATTGATTGACAGTCTGG(+ATATAT)GTACCACCACACACACAGACTTCAGGTATTT NGG 10 8 17 8 24 0 100 5 10
    240607035355_t36e TTAAGAAATACCTGAAGTCTGTGTGTGTGGTG(+ATATAT)GTACCCAGACTGTCAATCAATAAAGACCCAG NGG 10 8 17 8 24 0 100 5 10