Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607035445_bfos ATAAGGGCCCTGGACAGGCACAGATACTCTTG(+ATATAT)GATCCCCAGTGGAGATGGATAAGAACCTCAA NGG 10 8 17 8 24 0 100 5 10
    240607035443_w0at CCTCTTTGAGGTTCTTATCCATCTCCACTGGG(+ATATAT)GATCCAAGAGTATCTGTGCCTGTCCAGGGCC NGG 10 8 17 8 24 0 100 5 10
    240607035440_9p3p GGATGGACCATCCAGAGACTACCCCACCCGGG(+ATATAT)GATCCATCCCATAATCAGCCACCAAACCCAG NGG 10 8 17 8 24 0 100 5 10
    240607035438_nrmj AGTGTCTGGGTTTGGTGGCTGATTATGGGATG(+ATATAT)GATCCCCGGGTGGGGTAGTCTCTGGATGGTC NGG 10 8 17 8 24 0 100 5 10
    240607035437_6cya CTTGCTGCCAGGAGAGCTTGCTTGGGTATCAG(+ATATAT)GTACCATACCCCTACTGTGCAGAGGCTCTGG NGG 10 8 17 8 24 0 100 5 10
    240607035436_fq1y TCTTCCCAGAGCCTCTGCACAGTAGGGGTATG(+ATATAT)GTACCTGATACCCAAGCAAGCTCTCCTGGCA NGG 10 8 17 8 24 0 100 5 10
    240607035435_lb8l ATGACATGATCACCGCCTCACAGTCAGTGATG(+ATATAT)GTACCTTTGTTTTAGTTATGACCAGATAGCT NGG 10 8 17 8 24 0 100 5 10
    240607035434_fwkx ACCCCAGCTATCTGGTCATAACTAAAACAAAG(+ATATAT)GTACCATCACTGACTGTGAGGCGGTGATCAT NGG 10 8 17 8 24 0 100 5 10
    240607035433_nobk AGACTGGCAAATAAGTTGGCAGATGCACTTTG(+ATATAT)GTACCCTGTTCCTACTCTAGGCTTTAGAATG NGG 10 8 17 8 24 0 100 5 10
    240607035431_diaf TGCATCATTCTAAAGCCTAGAGTAGGAACAGG(+ATATAT)GTACCAAAGTGCATCTGCCAACTTATTTGCC NGG 10 8 17 8 24 0 100 5 10