Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607035531_83ay AAGAAGAAGAAGAAAAAAAGAATAAGACACTG(+ATATAT)GATCCTAAAGAAAACCGTGCCCAAGCCTAGA NGG 10 8 17 8 24 0 100 5 10
    240607035530_ob5v CGGGGTCTAGGCTTGGGCACGGTTTTCTTTAG(+ATATAT)GATCCAGTGTCTTATTCTTTTTTTCTTCTTC NGG 10 8 17 8 24 0 100 5 10
    240607035529_k7et GGTTGCAACCTTGAGAAAGCTCCAGTTACTGG(+ATATAT)GTACCCTGCTTATCGCTGCCTCCCAGGATTT NGG 10 8 17 8 24 0 100 5 10
    240607035527_ijxm CAGCAAAATCCTGGGAGGCAGCGATAAGCAGG(+ATATAT)GTACCCAGTAACTGGAGCTTTCTCAAGGTTG NGG 10 8 17 8 24 0 100 5 10
    240607035526_t96i ACCACTTGGTGGCTCACAACCATCTGTAATGG(+ATATAT)GATCCAATGCCCTCTTTAATGTGTCTGAAGA NGG 10 8 17 8 24 0 100 5 10
    240607035524_qtw2 CTCTGTCTTCAGACACATTAAAGAGGGCATTG(+ATATAT)GATCCCATTACAGATGGTTGTGAGCCACCAA NGG 10 8 17 8 24 0 100 5 10
    240607035523_gtdr GTTCATTCTGTCAGGTTCTTTCATACACAAGG(+ATATAT)GTACCTTCAGAAGAAACGGGCACCTATAACA NGG 10 8 17 8 24 0 100 5 10
    240607035521_uru1 TTCTTTGTTATAGGTGCCCGTTTCTTCTGAAG(+ATATAT)GTACCCTTGTGTATGAAAGAACCTGACAGAA NGG 10 8 17 8 24 0 100 5 10
    240607035520_ix4j GCCTTAGGAGTGAAGACAGCCAGGAGAGGCTG(+ATATAT)GTACCGGCCCGCCTCCCTCACGGAGTCTCCT NGG 10 8 17 8 24 0 100 5 10
    240607035518_bios TCTGCAGGAGACTCCGTGAGGGAGGCGGGCCG(+ATATAT)GTACCAGCCTCTCCTGGCTGTCTTCACTCCT NGG 10 8 17 8 24 0 100 5 10