Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607035631_x0x7 CTCAACTGAACCTTTTTTGTTGTCCTTTCTGG(+ATATAT)GATCCTCTGCAGTGTGGCCCAGGGACAGGCA NGG 10 8 17 8 24 0 100 5 10
    240607035630_7c0y CATGTTGCCTGTCCCTGGGCCACACTGCAGAG(+ATATAT)GATCCCAGAAAGGACAACAAAAAAGGTTCAG NGG 10 8 17 8 24 0 100 5 10
    240607035629_4w2q TGTTCTCCCCACCTGGATCTTTGGGCCTTAAG(+ATATAT)GTACCAGGCAGCGTTGAGAGCCTCATGAAGT NGG 10 8 17 8 24 0 100 5 10
    240607035627_bmag TTCCAACTTCATGAGGCTCTCAACGCTGCCTG(+ATATAT)GTACCTTAAGGCCCAAAGATCCAGGTGGGGA NGG 10 8 17 8 24 0 100 5 10
    240607035626_31oe GAATTCTATTACTATCTGGCTGTGCAGCTCAG(+ATATAT)GTACCAGATGTGTGCCAAGACTGTCCTGGAC NGG 10 8 17 8 24 0 100 5 10
    240607035625_3bvk CACGAGTCCAGGACAGTCTTGGCACACATCTG(+ATATAT)GTACCTGAGCTGCACAGCCAGATAGTAATAG NGG 10 8 17 8 24 0 100 5 10
    240607035623_3guu GAATTCTATTACTATCTGGCTGTGCAGCTCAG(+ATATAT)GTACCAGATGTGTGCCAAGACTGTCCTGGAC NGG 10 8 17 8 24 0 100 5 10
    240607035622_bdke CACGAGTCCAGGACAGTCTTGGCACACATCTG(+ATATAT)GTACCTGAGCTGCACAGCCAGATAGTAATAG NGG 10 8 17 8 24 0 100 5 10
    240607035621_y5mg ACAGTGGGTGTGAGCTCACATCCTGGCGTGGG(+ATATAT)GTACCAGAGGCAAGTTTCGACACTGTAGTGG NGG 10 8 17 8 24 0 100 5 10
    240607035618_96er ACGTGCCACTACAGTGTCGAAACTTGCCTCTG(+ATATAT)GTACCCCACGCCAGGATGTGAGCTCACACCC NGG 10 8 17 8 24 0 100 5 10