Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607035648_drzb TGGTCCTTCTCAGGCACTGTGGGGCCCTGGGG(+ATATAT)GATCCTCGTTCCTGGCCTTGCCCTTCCTGGC NGG 10 8 17 8 24 0 100 5 10
    240607035647_tyrh CCCTAGCCAGGAAGGGCAAGGCCAGGAACGAG(+ATATAT)GATCCCCCAGGGCCCCACAGTGCCTGAGAAG NGG 10 8 17 8 24 0 100 5 10
    240607035645_hr11 AGAGGGCACCGGTGGGAGTCCTGGGGTTGGTG(+ATATAT)GTACCTTCATGTGCTCAGCATCTCGCCAGCC NGG 10 8 17 8 24 0 100 5 10
    240607035644_444a GTCAAGGCTGGCGAGATGCTGAGCACATGAAG(+ATATAT)GTACCACCAACCCCAGGACTCCCACCGGTGC NGG 10 8 17 8 24 0 100 5 10
    240607035643_akn2 ATGAGGCCAGGAGAGGGTACTGGATGGTACGG(+ATATAT)GATCCCCTGGACCTGGAGATACAGATGGTTC NGG 10 8 17 8 24 0 100 5 10
    240607035640_3sd3 GCTTAGAACCATCTGTATCTCCAGGTCCAGGG(+ATATAT)GATCCCGTACCATCCAGTACCCTCTCCTGGC NGG 10 8 17 8 24 0 100 5 10
    240607035639_g1mu GCACCTCAGGGAGATAGTGCCTGGTGACTTTG(+ATATAT)GATCCTGAGTTTGAAATCAGGTGTAGAGAGT NGG 10 8 17 8 24 0 100 5 10
    240607035638_ii4c ACTAGACTCTCTACACCTGATTTCAAACTCAG(+ATATAT)GATCCAAAGTCACCAGGCACTATCTCCCTGA NGG 10 8 17 8 24 0 100 5 10
    240607035635_8sgk TAGCTCTGCTGAACCAGCAGCCCCCTCCCCAG(+ATATAT)GATCCTCAGGAAGGAGGGGGCACATGGCTGG NGG 10 8 17 8 24 0 100 5 10
    240607035633_r5er TCGGTCCAGCCATGTGCCCCCTCCTTCCTGAG(+ATATAT)GATCCTGGGGAGGGGGCTGCTGGTTCAGCAG NGG 10 8 17 8 24 0 100 5 10