Enter the sequence


Example input
More Parameters

Example for substitution:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTGGCGCGA(/T)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for insertion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGCGCTG(+ATT)GCGCGAGGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Example for deletion:

CGCCGCCGCCTCTACTGGGGCTTCTTCTCGGGCCGCGGCCGCGTCAAGCCGGGGGGGC(-GCTGGCGCGA)GGCCGCCTGGCAACTCTGCGACTACTACCTGCC

Above three examples above represent the input formats of three different editing types.

  • The bases to be edited are marked with a pair of parentheses "()".

  • Substitution is indicated by backslash "/", and the example is used to replace A by T.

  • Insertion is indicated by plus sign "+", and the example indicates that ATT will be inserted at the parenthesis position

  • Deletion is indicated by minus sign "-", and the example indicates that GCTGGCGCGA will be deleted at the parenthesis position.

    • PAM Maximum Edit-to-Nick Distance
    Maximum PBS Length Maximum RTT Length
    Minimum PBS Length Minimum RTT Length
    Number of Optimized pegRNAs Assembly
    Minimum Nick-to-Nick Distance Maximum Nick-to-Nick Distance
    Minimum Downstream Homology

    Use an example: Substitution Insertion Deletion
    Example for FASTA file History
    UID Input sequence PAM CUT SIZE MIN PBS MAX PBS MIN RT MAX RT MIN DIS SGRNA MAX DIS SGRNA HOMOLOGY Number show
    240607035753_5hhm AGACGCATGCGCACTGGCTTGCAAGGGCTCTG(+ATATAT)GATCCGACTTCACTTCTTATTAGCCTGAGCA NGG 10 8 17 8 24 0 100 5 10
    240607035752_ozf6 GGTACTGCTCAGGCTAATAAGAAGTGAAGTCG(+ATATAT)GATCCAGAGCCCTTGCAAGCCAGTGCGCATG NGG 10 8 17 8 24 0 100 5 10
    240607035750_03rq GCAGCCCGGCTAGTCCTTGAGATCCCGGCCCG(+ATATAT)GATCCTGGAGCAGGCGTGCACAGGCCAAAGC NGG 10 8 17 8 24 0 100 5 10
    240607035748_v1uh TGCCAGCTTTGGCCTGTGCACGCCTGCTCCAG(+ATATAT)GATCCGGGCCGGGATCTCAAGGACTAGCCGG NGG 10 8 17 8 24 0 100 5 10
    240607035747_oeg5 AGGATTTGAACTTGGGCCCTCTGTAAGAAGAG(+ATATAT)GATCCATTCTTAACTCCTGGACCATCTCATT NGG 10 8 17 8 24 0 100 5 10
    240607035746_uqrq AGGCTAATGAGATGGTCCAGGAGTTAAGAATG(+ATATAT)GATCCTCTTCTTACAGAGGGCCCAAGTTCAA NGG 10 8 17 8 24 0 100 5 10
    240607035743_gssq ACGAGTGCTGAGGATCTCAGCAGGTGGATGGG(+ATATAT)GATCCCAGGGATGCAAGAGCTTTCACCCTCT NGG 10 8 17 8 24 0 100 5 10
    240607035741_50z1 GGTGCAGAGGGTGAAAGCTCTTGCATCCCTGG(+ATATAT)GATCCCCATCCACCTGCTGAGATCCTCAGCA NGG 10 8 17 8 24 0 100 5 10
    240607035740_onw1 AAAACAAAAAACCCCAAAACTTCCTACTGCAG(+ATATAT)GTACCCCTTCATTACAAAAGTATGCAAACCA NGG 10 8 17 8 24 0 100 5 10
    240607035738_efsp TGAGATGGTTTGCATACTTTTGTAATGAAGGG(+ATATAT)GTACCTGCAGTAGGAAGTTTTGGGGTTTTTT NGG 10 8 17 8 24 0 100 5 10